Structure of PDB 4v6m Chain BW

Receptor sequence

>4v6mBW (length=84) Species: 83333 (Escherichia coli K-12)

AHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANV
GCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE

3D structure

• PDB: 4v6m Cryo-EM structure of the ribosome-SecYE complex in the membrane environment.
• Chain: BW
• Resolution: 7.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BW	H2 K3 K4	H2 K3 K4
BS02	rna	BW	G72 P73 K74	G72 P73 K74
BS03	rna	BW	A1 H2 K4 G7 T9 R10 N11 G12 R13 D14 S15 E16 R19 K23 F25 E28 V30 L31 R38 Q39 R40 G41 T42 G53 R54 D55 T57 F59 K61 F68 E69 R76	A1 H2 K4 G7 T9 R10 N11 G12 R13 D14 S15 E16 R19 K23 F25 E28 V30 L31 R38 Q39 R40 G41 T42 G53 R54 D55 T57 F59 K61 F68 E69 R76

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0043022 ribosome binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0001558 regulation of cell growth
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042256 cytosolic ribosome assembly
• GO:0090070 positive regulation of ribosome biogenesis
• GO:1902626 assembly of large subunit precursor of preribosome

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v6m, PDBe:4v6m, PDBj:4v6m
• PDBsum: 4v6m
• PubMed: 21499241
• UniProt: P0A7L8|RL27_ECOLI Large ribosomal subunit protein bL27 (Gene Name=rpmA)