Structure of PDB 8oit Chain BT

Receptor sequence

>8oitBT (length=291) Species: 9606 (Homo sapiens)

QGGGARALDLLRGLPRVSLANLKPNPGSKKPERRPRGRRRGRKCGRGHKG
ERQRGTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYKPLSLNRLQYLI
DLGRVDPSQPIDLTQLVNGRGVTIQPLKRDYGVQLVEEGADTFTAKVNIE
VQLASELAIAAIEKNGGVVTTAFYDPRSLDIVCKPVPFFLRGQPIPKRML
PPEELVPYYTDAKNRGYLADPAKFPEARLELARKYGYILPDITKDELFKM
LCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS

3D structure

• PDB: 8oit Molecular basis of translation termination at noncanonical stop codons in human mitochondria.
• Chain: BT
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BT	L24 A25 L27 P29 N30 G32 S33 K34 K35 E37 R38 R39 R41 G42 R44 R45 G46 R47 K48 C49 G50 R51 H53 K54 G55 E56 R57 Q58 R59 T61 R62 P63 R64 F67 E68 G69 G70 Q71 T72 Y75 I76 R77 P79 K80 H87 S88 R100 Y103 R109 T128 Q130 L132 K133 R134 K189 R196	L19 A20 L22 P24 N25 G27 S28 K29 K30 E32 R33 R34 R36 G37 R39 R40 G41 R42 K43 C44 G45 R46 H48 K49 G50 E51 R52 Q53 R54 T56 R57 P58 R59 F62 E63 G64 G65 Q66 T67 Y70 I71 R72 P74 K75 H82 S83 R95 Y98 R104 T123 Q125 L127 K128 R129 K184 R191

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0006412 translation
• GO:0032543 mitochondrial translation
• GO:1990830 cellular response to leukemia inhibitory factor

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005762 mitochondrial large ribosomal subunit
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8oit, PDBe:8oit, PDBj:8oit
• PDBsum: 8oit
• PubMed: 37141370
• UniProt: Q9P015|RM15_HUMAN Large ribosomal subunit protein uL15m (Gene Name=MRPL15)