Structure of PDB 4wf1 Chain BS

Receptor sequence

>4wf1BS (length=110) Species: 83333 (Escherichia coli K-12)

METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKV
LESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRT
SHITVVVSDR

3D structure

• PDB: 4wf1 Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome.
• Chain: BS
• Resolution: 3.09 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BS	I4 A5 K6 H7 R8 H9 R11 S13 Q15 K16 R18 R25 K41 K42 K49 S53 A56 N57 H60 N61 D77 E78 P80 M82 K83 R84 M86 P87 R88 A89 K90 G91 R92 A93 D94 R95 I96 L97 K98 H102	I4 A5 K6 H7 R8 H9 R11 S13 Q15 K16 R18 R25 K41 K42 K49 S53 A56 N57 H60 N61 D77 E78 P80 M82 K83 R84 M86 P87 R88 A89 K90 G91 R92 A93 D94 R95 I96 L97 K98 H102

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042255 ribosome assembly
• GO:0042256 cytosolic ribosome assembly
• GO:0046677 response to antibiotic
• GO:1902626 assembly of large subunit precursor of preribosome

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wf1, PDBe:4wf1, PDBj:4wf1
• PDBsum: 4wf1
• PubMed: 25368144
• UniProt: P61175|RL22_ECOLI Large ribosomal subunit protein uL22 (Gene Name=rplV)