Structure of PDB 4w2f Chain BS

Receptor sequence

>4w2fBS (length=110) Species: 300852 (Thermus thermophilus HB8)

RLTAYERRKFRVRNRIKRTGRLRLSVFRSLKHIYAQIIDDEKGVTLVSAS
SLALKLKGNKTEVARQVGRALAEKALALGIKQVAFDRGPYKYHGRVKALA
EGAREGGLEF

3D structure

• PDB: 4w2f Amicoumacin a inhibits translation by stabilizing mRNA interaction with the ribosome.
• Chain: BS
• Resolution: 2.4 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BS	R3 L4 R9 R10 F12 R13 N16 R17 I18 T21 R23 R89 Y94 R106 F112	R1 L2 R7 R8 F10 R11 N14 R15 I16 T19 R21 R87 Y92 R104 F110
BS02	rna	BS	R15 R25 F29 R30 S31 L32 Y36 Q38 G45 V46 T47 S50 L54 K62 T63 Y92 K93 H95 G96 R97	R13 R23 F27 R28 S29 L30 Y34 Q36 G43 V44 T45 S48 L52 K60 T61 Y90 K91 H93 G94 R95
BS03	MG	BS	R15 V28 D88 G90	R13 V26 D86 G88
BS04	MG	BS	I35 K62 A66 R97	I33 K60 A64 R95

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4w2f, PDBe:4w2f, PDBj:4w2f
• PDBsum: 4w2f
• PubMed: 25306919
• UniProt: Q5SHQ4|RL18_THET8 Large ribosomal subunit protein uL18 (Gene Name=rplR)