Structure of PDB 8ova Chain BQ

Receptor sequence

>8ovaBQ (length=203) Species: 5702 (Trypanosoma brucei brucei)

GSFMYLNELWKKKSSDVMRFIQRVRAWEFRHQHTIVRLRRPTRPEKARMV
GYKTKQGYVVFRVRVRRGGRKRPVHKGITYGKPNTAGVLGRKLNKNNRVV
AEQRLGKKYGNLRVLNSYWVNADSTFLWYEVVAVDPMHRAIRRDPRINWI
VNAVHKHREQRGLTSAGRKHRGLRQKGHKASKLRPSRRAAWRRNNRMVFL
RKR

3D structure

• PDB: 8ova A single pseudouridine on rRNA regulates ribosome structure and function in the mammalian parasite Trypanosoma brucei.
• Chain: BQ
• Resolution: 2.47 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BQ	G19 F21 K29 K31 H51 T52 R57 R58 R61 K64 R66 M67 K71 T72 K73 Q74 R84 R85 R88 R90 H93 Y98 K100 P101 N102 G108 K110 L111 N112 K113 N114 R122 K125 N129 N134 M155 R157 W167 K174 H175 R179 S183 R186 K187 H188 R189 K194 G195 H196 K197 K200 R202 S204 R205 W209 R210 N212 R221	G1 F3 K11 K13 H33 T34 R39 R40 R43 K46 R48 M49 K53 T54 K55 Q56 R66 R67 R70 R72 H75 Y80 K82 P83 N84 G90 K92 L93 N94 K95 N96 R104 K107 N111 N116 M137 R139 W149 K156 H157 R161 S165 R168 K169 H170 R171 K176 G177 H178 K179 K182 R184 S186 R187 W191 R192 N194 R203
BS02	rna	BQ	R48 R84 K89 P91 H93 K94 L107	R30 R66 K71 P73 H75 K76 L89
BS03	rna	BQ	R55 Y127 N129	R37 Y109 N111

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ova, PDBe:8ova, PDBj:8ova
• PDBsum: 8ova
• PubMed: 37985661
• UniProt: Q38CY7