Structure of PDB 4wf1 Chain BQ

Receptor sequence

>4wf1BQ (length=117) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

ARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQYAYRDRR
QRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVF
DKVAFTALVEKAKAALA

3D structure

PDB

4wf1 Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome.

Chain

Resolution

3.09 Å

3D
structure

Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BQ	A2 R3 V4 K5 R6 R11 R13 H14 K15 K16 K22 G23 Y24 Y25 G26 A27 R28 R30 V31 R33 V34 F36 Q37 K41 A42 Y45 A46 Y47 R48 D49 R50 R51 Q52 R53 K54 R55 Q56 F57 R58 Q59 W61 I62 N66 R70 S75 Y76 S77 N81 D91 R92 K93	A1 R2 V3 K4 R5 R10 R12 H13 K14 K15 K21 G22 Y23 Y24 G25 A26 R27 R29 V30 R32 V33 F35 Q36 K40 A41 Y44 A45 Y46 R47 D48 R49 R50 Q51 R52 K53 R54 Q55 F56 R57 Q58 W60 I61 N65 R69 S74 Y75 S76 N80 D90 R91 K92

Gene Ontology

	Molecular Function
GO:0000900	mRNA regulatory element binding translation repressor activity
GO:0003729	mRNA binding
GO:0003735	structural constituent of ribosome
GO:0019843	rRNA binding
GO:0070180	large ribosomal subunit rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation
GO:0017148	negative regulation of translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

External links

PDB	RCSB:4wf1, PDBe:4wf1, PDBj:4wf1
PDBsum	4wf1
PubMed	25368144
UniProt	P0A7L3\|RL20_ECOLI Large ribosomal subunit protein bL20 (Gene Name=rplT)