Structure of PDB 7o81 Chain BK

Receptor sequence

>7o81BK (length=39) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

IPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPM

3D structure

• PDB: 7o81 Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome.
• Chain: BK
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BK	T4355 D4359 T4371 M4375 W4376 Y4379 S4382 C4383 D4384 Q4385 L4386 R4387 E4388 P4389 M4390	T4 D8 T20 M24 W25 Y28 S31 C32 D33 Q34 L35 R36 E37 P38 M39

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0005524 ATP binding
• GO:0008270 zinc ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:7o81, PDBe:7o81, PDBj:7o81
• PDBsum: 7o81
• PubMed: 34029205
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)