Structure of PDB 8evt Chain BI

Receptor sequence

>8evtBI (length=188) Species: 4932 (Saccharomyces cerevisiae)

GISRDSRHKRSATGAKRAQFRKKRKFELGRQPANTKIGAKRIHSVRTRGG
NKKYRALRIETGNFSWASEGISKKTRIAGVVYHPSNNELVRTNTLTKAAI
VQIDATPFRQWFEAHYGQTLGKKSKNAERKWAARAASAKIESSVESQFSA
GRLYACISSRPGQSGRCDGYILEGEELAFYLRRLTAKK

3D structure

• PDB: 8evt Regulation of translation by ribosomal RNA pseudouridylation.
• Chain: BI
• Resolution: 2.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BI	G2 R5 D6 S7 R8 H9 K10 R11 A13 T14 G15 A16 F21 R22 K23 K24 R25 K26 F27 L29 G30 R31 P33 A34 K41 R42 I43 R47 T48 R49 G50 N52 K54 R56 N64 S66 A68 S73 K75 S86 N87 T97 K98 A99 K137 N138 A139 R141 K142 R146 S170 S171 R172 G174 Q175 S176 R178 D180	G1 R4 D5 S6 R7 H8 K9 R10 A12 T13 G14 A15 F20 R21 K22 K23 R24 K25 F26 L28 G29 R30 P32 A33 K40 R41 I42 R46 T47 R48 G49 N51 K53 R55 N63 S65 A67 S72 K74 S85 N86 T96 K97 A98 K125 N126 A127 R129 K130 R134 S158 S159 R160 G162 Q163 S164 R166 D168
BS02	rna	BI	R77 R92 A162 R164 K200	R76 R91 A150 R152 K188
BS03	U	BI	H44 L58	H43 L57
BS04	C	BI	R42 L58 R59	R41 L57 R58
BS05	A	BI	R42 R59	R41 R58
BS06	A	BI	I3 Q32	I2 Q31

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8evt, PDBe:8evt, PDBj:8evt
• PDBsum: 8evt
• PubMed: 37595043
• UniProt: P0CX39|RS8A_YEAST Small ribosomal subunit protein eS8A (Gene Name=RPS8A)