Structure of PDB 5ju8 Chain BF

Receptor sequence

>5ju8BF (length=177) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

PDB

5ju8 A combined cryo-EM and molecular dynamics approach reveals the mechanism of ErmBL-mediated translation arrest.

Chain

Resolution

3.6 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	BF	S73 V74 A75	S72 V73 A74
BS02	rna	BF	T35 N37 G39 G41 T68 A70 R71 K72 F77 I79 I85 S121 D123 R125 N127 M130 G131 G151 T155 T157	T34 N36 G38 G40 T67 A69 R70 K71 F76 I78 I84 S120 D122 R124 N126 M129 G130 G150 T154 T156
BS03	rna	BF	S24 V25 M26 Q63 K64 L66 K69 K88 V89 T90 R92	S23 V24 M25 Q62 K63 L65 K68 K87 V88 T89 R91

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome
GO:0008097	5S rRNA binding
GO:0019843	rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:5ju8, PDBe:5ju8, PDBj:5ju8
PDBsum	5ju8
PubMed	27380950
UniProt	P62399\|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)

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