Structure of PDB 4wf1 Chain BF

Receptor sequence

>4wf1BF (length=177) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

PDB

4wf1 Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome.

Chain

Resolution

3.09 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BF	K33 T35 N37 M38 G39 V40 G41 T68 A70 R71 F77 I79 I85 K88 S121 D123 R125 S129 G131 R133 G151 L152 D153 T155	K32 T34 N36 M37 G38 V39 G40 T67 A69 R70 F76 I78 I84 K87 S120 D122 R124 S128 G130 R132 G150 L151 D152 T154
BS02	rna	BF	M26 Q27 Q63 K64 L66 K69 V89 T90 R92	M25 Q26 Q62 K63 L65 K68 V88 T89 R91

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome
GO:0008097	5S rRNA binding
GO:0019843	rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:4wf1, PDBe:4wf1, PDBj:4wf1
PDBsum	4wf1
PubMed	25368144
UniProt	P62399\|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)

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