Structure of PDB 4v89 Chain BF

Receptor sequence

>4v89BF (length=177) Species: 562 (Escherichia coli)

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

• PDB: 4v89 Crystal structure of release factor RF3 trapped in the GTP state on a rotated conformation of the ribosome.
• Chain: BF
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BF	N36 M37 G38 V39 G40 T67 A69 R70 F76 I78 I84 K87 S120 D122 R124 S128 G130 R149 G150 L151	N36 M37 G38 V39 G40 T67 A69 R70 F76 I78 I84 K87 S120 D122 R124 S128 G130 R149 G150 L151
BS02	rna	BF	S23 M25 Q26 K63 L65 K68 V88 T89 R91 K160	S23 M25 Q26 K63 L65 K68 V88 T89 R91 K160

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v89, PDBe:4v89, PDBj:4v89
• PDBsum: 4v89
• PubMed: 22187675
• UniProt: P62399|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)