Structure of PDB 6gaz Chain BC

Receptor sequence

>6gazBC (length=571) Species: 9606 (Homo sapiens)

DKVRKNKDAVRRPQADPALLTPRSPVVTIMGHVDHGKTTLLDKFRKTQVA
AVETGGITQHIGAFLVSLPSGEKITFLDTPGHAAFSAMRARGAQVTDIVV
LVVAADDGVMKQTVESIQHAKDAQVPIILAVNKCDKAEADPEKVKKELLA
YDVVCEDYGGDVQAVPVSALTGDNLMALAEATVALAEMLELKADPNGPVE
GTVIESFTDKGRGLVTTAIIQRGTLRKGSVLVAGKCWAKVRLMFDENGKT
IDEAYPSMPVGITGWRDLPSAGEEILEVESEPRAREVVDWRKYEQEQEKG
QEDLKIIEEKRKEHKEAHQKAREKYGHLLWKKRSILRFLERKEQIPLKPK
EKRERDSNVLSVIIKGDVDGSVEAILNIIDTYDASHECELELVHFGVGDV
SANDVNLAETFDGVIYGFNVNAGNVIQQSAAKKGVKIKLHKIIYRLVEDL
QEELSSRLPCAVEEHPVGEASILATFSVTEGKKKVPVAGCRVQKGQLEKQ
KKFKLTRNGHVIWKGSLTSLKHHKDDISIVKTGMDCGLSLDEDNMEFQVG
DRIVCYEEKQIQAKTSWDPGF

3D structure

• PDB: 6gaz Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM.
• Chain: BC
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BC	K158 K366 G367 G369 R397 H470 K471 H474 R478 W486 S490 F494 S557 N559	K2 K210 G211 G213 R241 H314 K315 H318 R322 W330 S334 F338 S401 N403
BS02	rna	BC	V634 T635 E636 V643 S675 K677 K680 S695	V478 T479 E480 V487 S519 K521 K524 S539
BS03	GSP	BC	D190 G192 K193 T194 T195 T214 G237 N288 K289 D291 S324 A325 L326	D34 G36 K37 T38 T39 T58 G81 N132 K133 D135 S168 A169 L170
BS04	MG	BC	T194 T214	T38 T58
BS05	FME	BC	G645 H678 H679 D691	G489 H522 H523 D535

Gene Ontology

Molecular Function

• GO:0003743 translation initiation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding

Biological Process

• GO:0006413 translational initiation

External links

• PDB: RCSB:6gaz, PDBe:6gaz, PDBj:6gaz
• PDBsum: 6gaz
• PubMed: 30089917
• UniProt: P46199|IF2M_HUMAN Translation initiation factor IF-2, mitochondrial (Gene Name=MTIF2)