Structure of PDB 6gaw Chain BC

Receptor sequence

>6gawBC (length=571) Species: 9606 (Homo sapiens)

DKVRKNKDAVRRPQADPALLTPRSPVVTIMGHVDHGKTTLLDKFRKTQVA
AVETGGITQHIGAFLVSLPSGEKITFLDTPGHAAFSAMRARGAQVTDIVV
LVVAADDGVMKQTVESIQHAKDAQVPIILAVNKCDKAEADPEKVKKELLA
YDVVCEDYGGDVQAVPVSALTGDNLMALAEATVALAEMLELKADPNGPVE
GTVIESFTDKGRGLVTTAIIQRGTLRKGSVLVAGKCWAKVRLMFDENGKT
IDEAYPSMPVGITGWRDLPSAGEEILEVESEPRAREVVDWRKYEQEQEKG
QEDLKIIEEKRKEHKEAHQKAREKYGHLLWKKRSILRFLERKEQIPLKPK
EKRERDSNVLSVIIKGDVDGSVEAILNIIDTYDASHECELELVHFGVGDV
SANDVNLAETFDGVIYGFNVNAGNVIQQSAAKKGVKIKLHKIIYRLVEDL
QEELSSRLPCAVEEHPVGEASILATFSVTEGKKKVPVAGCRVQKGQLEKQ
KKFKLTRNGHVIWKGSLTSLKHHKDDISIVKTGMDCGLSLDEDNMEFQVG
DRIVCYEEKQIQAKTSWDPGF

3D structure

• PDB: 6gaw Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM.
• Chain: BC
• Resolution: 3.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BC	M266 R601 K680 D681 T688	M110 R445 K524 D525 T532
BS02	rna	BC	K366 G367 K395 R397 L398 H470 K471 H474 L485 W486 K487 S490 S557 N559	K210 G211 K239 R241 L242 H314 K315 H318 L329 W330 K331 S334 S401 N403
BS03	rna	BC	T635 E636 V643 K677 K680 S695	T479 E480 V487 K521 K524 S539
BS04	GSP	BC	V189 D190 G192 K193 T194 T195 T214 G237 N288 K289 D291 A325 L326	V33 D34 G36 K37 T38 T39 T58 G81 N132 K133 D135 A169 L170
BS05	MG	BC	T194 T214	T38 T58
BS06	FME	BC	G645 H678 H679 D691	G489 H522 H523 D535

Gene Ontology

Molecular Function

• GO:0003743 translation initiation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding

Biological Process

• GO:0006413 translational initiation

External links

• PDB: RCSB:6gaw, PDBe:6gaw, PDBj:6gaw
• PDBsum: 6gaw
• PubMed: 30089917
• UniProt: P46199|IF2M_HUMAN Translation initiation factor IF-2, mitochondrial (Gene Name=MTIF2)