Structure of PDB 4wf1 Chain BC

Receptor sequence

>4wf1BC (length=271) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITT
RHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGER
RYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKG
GQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHM
LRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQ
TKGKKTRSNKRTDKFIVRRRS

3D structure

PDB

4wf1 Negamycin induces translational stalling and miscoding by binding to the small subunit head domain of the Escherichia coli ribosome.

Chain

Resolution

3.09 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	BC	K7 P8 T9 P11 R13 R14 H15 K18 G27 K28 E35 K36 K39 S40 G42 R43 N44 N45 N46 G47 R48 I49 T50 T51 R52 H53 G56 H58 K59 Q60 A61 Y62 R63 I74 Y83 P85 N86 R87 D98 G99 R101 K147 K150 Q153 L154 A155 R156 S157 A158 G159 T160 Y171 R177 S178 E180 R182 A198 H200 M201 V204 G206 K207 A208 G209 R212 W213 R214 R217 P218 T219 V220 R221 G222 T223 A224 M225 N226 V228 D229 H230 P231 H232 G235 E236 G237 R238 N239 F240 G241 K242 H243 P244 P247 W248 T252 K253 G254 K255 K256 T257 R258 R262 T263 R270 R271	K6 P7 T8 P10 R12 R13 H14 K17 G26 K27 E34 K35 K38 S39 G41 R42 N43 N44 N45 G46 R47 I48 T49 T50 R51 H52 G55 H57 K58 Q59 A60 Y61 R62 I73 Y82 P84 N85 R86 D97 G98 R100 K146 K149 Q152 L153 A154 R155 S156 A157 G158 T159 Y170 R176 S177 E179 R181 A197 H199 M200 V203 G205 K206 A207 G208 R211 W212 R213 R216 P217 T218 V219 R220 G221 T222 A223 M224 N225 V227 D228 H229 P230 H231 G234 E235 G236 R237 N238 F239 G240 K241 H242 P243 P246 W247 T251 K252 G253 K254 K255 T256 R257 R261 T262 R269 R270

Gene Ontology

	Molecular Function
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016740	transferase activity
GO:0019843	rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation
GO:0032297	negative regulation of DNA-templated DNA replication initiation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0015934	large ribosomal subunit
GO:0022625	cytosolic large ribosomal subunit
GO:1990082	DnaA-L2 complex
GO:1990904	ribonucleoprotein complex

View graph for
Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:4wf1, PDBe:4wf1, PDBj:4wf1
PDBsum	4wf1
PubMed	25368144
UniProt	P60422\|RL2_ECOLI Large ribosomal subunit protein uL2 (Gene Name=rplB)

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