Structure of PDB 8oru Chain BBB

Receptor sequence

>8oruBBB (length=459) Species: 523846 (Methanothermus fervidus DSM 2088)

GETKKMICLVDGEHYFPVVKDSIEILDDLEHIDVVAVVFIGGTEKLQIED
PKEYSEKLGKPVFFGPDPKKIPYDVIKKCVKKYNADIVMDLSDEPVVDYT
KRFRIASIVLKEGAVYQGADFKFEPLTEYDVLEKPSIKIIGTGKRIGKTA
VSAYAARVIHKHKYNPCVVAMGRGGPREPEIVEGNKIEITAEYLLEQADK
GVHAASDHWEDALMSRILTVGCRRCGGGMLGDTFITNVKRGAEIANKLDS
DFVIMEGSGAAIPPVKTNRQIVTVGANQPMININNFFGPFRIGLADLVII
TMCEEPMATTEKIKKVEKFIKEINPSANVIPTVFRPKPVGNVEGKKVLFA
TTAPKVVVGKLVNYLESKYGCDVVGVTPHLSNRPLLRRDLKKYINKADLM
LTELKAAAVDVATRVAIEAGLDVVYCDNIPVVIDESYGNIDDAIIEVVEM
AIDDFKNNR

3D structure

• PDB: 8oru Structural characterization of a novel cyclic 2,3-diphosphoglycerate synthetase involved in extremolyte production in the archaeon Methanothermus fervidus
• Chain: BBB
• Resolution: 2.23 Å

Enzymatic activity

• Enzyme Commision number: 6.5.1.9: cyclic 2,3-diphosphoglycerate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	DG2	BBB	K356 H380 R389	K355 H379 R388
BS02	ADP	BBB	R146 I147 G148 K149 T150 A151 M303 F335 T353 A354 V358 E404 K406 N429	R145 I146 G147 K148 T149 A150 M302 F334 T352 A353 V357 E403 K405 N428
BS03	MG	BBB	T150 D208	T149 D207
BS04	MG	BBB	D28 L30 I33	D27 L29 I32
BS05	PO4	BBB	K145 R146 K149 R174 S259 G260	K144 R145 K148 R173 S258 G259

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0036356 cyclic 2,3-diphosphoglycerate synthetase activity

Biological Process

• GO:0006094 gluconeogenesis
• GO:1901369 cyclic 2,3-bisphospho-D-glycerate biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8oru, PDBe:8oru, PDBj:8oru
• PDBsum: 8oru
• PubMed: 38045033
• UniProt: O93732|CPGS_METFV Cyclic 2,3-diphosphoglycerate synthetase (Gene Name=cpgS)