Structure of PDB 6zic Chain BBB

Receptor sequence

>6zicBBB (length=714) Species: 10116 (Rattus norvegicus)

HMAEYLRLPHSLAMIRLCNPPVNAVSPTVIREVRNGLQKAGSDHTVKAIV
ICGANGNFCAGADIHGFSAFTPGLALGSLVDEIQRYQKPVLAAIQGVALG
GGLELALGCHYRIANAKARVGLPEVTLGILPGARGTQLLPRVVGVPVALD
LITSGKYLSADEALRLGILDAVVKSDPVEEAIKFAQKIIDKPIEPRRIFN
KPVPSLPNMDSVFAEAIAKVRKQYPGVLAPETCVRSIQASVKHPYEVGIK
EEEKLFMYLRASGQAKALQYAFFAEKSANKWSTPSGASWKTASAQPVSSV
GVLGLGTMGRGIAISFARVGISVVAVESDPKQLDAAKKIITFTLEKEASR
AHQSAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAELSALCKPGA
FLCTNTSALNVDDIASSTDRPQLVIGTHFFSPAHVMRLLEVIPSRYSSPT
TIATVMSLSKKIGKIGVVVGNCYGFVGNRMLAPYYNQGFFLLEEGSKPED
VDGVLEEFGFKMGPFRVSDLAGLDVGWKIRKGQGLTGPSLPPGTPVRKRG
NSRYSPLGDMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLSTFLSQYR
EVHHIEQRTISKEEILERCLYSLINEAFRILEEGMAARPEHIDVIYLHGY
GWPRHKGGPMFYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYLRRLVAQ
GSPPLKEWQSLAGP

3D structure

• PDB: 6zic CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE-1 (RPMFE1) COMPLEXED WITH 3S-HYDROXYBUTANOYL-COA AND NADH
• Chain: BBB
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): A61 F66 G76 G100 E103 P122 E123 G131 K249 S410 H431 E443 N481
• Catalytic site (residue number reindexed from 1): A62 F67 G77 G101 E104 P123 E124 G132 K250 S407 H428 E440 N478
• Enzyme Commision number: 1.1.1.35: 3-hydroxyacyl-CoA dehydrogenase.
  4.2.1.17: enoyl-CoA hydratase.
  5.3.3.8: Delta(3)-Delta(2)-enoyl-CoA isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	3HC	BBB	P20 V21 A59 G60 A61 D62 I63 F66 L98 G99 G100 E103 P122 E123 L126 Y156 F271 K275 K279	P21 V22 A60 G61 A62 D63 I64 F67 L99 G100 G101 E104 P123 E124 L127 Y157 F272 K276 K280
BS02	NAI	BBB	G303 G305 T306 M307 E326 S327 Q331 A380 V381 F382 E383 K388 N408 S410 H431	G304 G306 T307 M308 E327 S328 Q332 A377 V378 F379 E380 K385 N405 S407 H428

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity
• GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity
• GO:0004300 enoyl-CoA hydratase activity
• GO:0016491 oxidoreductase activity
• GO:0016509 long-chain-3-hydroxyacyl-CoA dehydrogenase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0016829 lyase activity
• GO:0016853 isomerase activity
• GO:0016863 intramolecular oxidoreductase activity, transposing C=C bonds
• GO:0019899 enzyme binding
• GO:0070403 NAD+ binding

Biological Process

• GO:0006631 fatty acid metabolic process
• GO:0006635 fatty acid beta-oxidation

Cellular Component

• GO:0005777 peroxisome
• GO:0005829 cytosol

External links

• PDB: RCSB:6zic, PDBe:6zic, PDBj:6zic
• PDBsum: 6zic
• UniProt: P07896|ECHP_RAT Peroxisomal bifunctional enzyme (Gene Name=Ehhadh)