Structure of PDB 7nz2 Chain B4

Receptor sequence

>7nz2B4 (length=1467) Species: 230089 (Photorhabdus thracensis)

IERGKFRSLTLVNWNGFFARTFDLDELVTTLSGGNGAGKSTTMAAFVTAL
IPDLTLLHFRNTTEAGATSGSRDKGLHGKLRAGVCYSTLDVINSRHQRVV
VGVRLQQVAGRDRKVDIKPFMIQGLPTAIQPTQLLTENVGERQARVLPLN
ELKDRLDEMEGVQFKQFNSITDYHAQMFDLGVIPKRLRSASDRSKFYRLI
EASLYGGISSAITRSLRDYLLPENSGVRKAFQDMEAALRENRITLEAIRV
TQSDRDLFKHLITEATSYVSADYMRHANERRTHLDEALALRGELFGSHKQ
LATEQYRHVEMARELAEQSGASSDLETDHQAASDHLNLVQTAMRQQEKID
RYQVDLEELSYRLEEQTDVVEEAGELQAEYEARTEATEQEVDELKSQLAD
YQQALDVQQTRAIQYQQALQALERARELCRLPDLSVDNAEEWLETFQAKE
QQATEALLALEQKLSVADAAHNQFEQAYQLVKNIVGETSRSEAWQSAREL
LRDWPSQRHLADRVQPLRMRLSELEQRLNNQQNAERLLSEFCKRQGRQYQ
AEDLEALQNELEARQEALSLSVNEGGERRMEMRQELEQLKQKIQSLTARA
PVWLAAQDTLNQLCEQSGETLASSNDVTEYMQQLLEREREATVERDEVAA
QKRELEKQIERLSQPSGAEDSRMIALAERFGGVLLSEIYDDITIDDAPYF
SALYGPARHGIVVPDLSLVRPHLETLEDCPEDLYLIEGDPQSFDDSVFNA
EEQTNAVLVKSSDRQWRYSRYPELPLFGRAARENRLEALNLERDALAERY
ATLSFDVQKIQRAHQAFSQFVGKHLSVAFDTDPEAEIRELRQRHTELERE
VSRFEDQTQQQRQQYAQAKESLTTLNRLIPQVTLLLDETLIDRVEEVREE
MDEAQEAARFLQQHGSALTKLEPMVAVLQSDPQQHEQLQQDYETAKHSQH
QAKQQAFALVEIVQRRVHFSYSDSAGMLSENADLNDKLRQRLEHAESDRS
RAREQLRQQQAQYSQFNQVLASLKSSYETKQDMLKELLQEMKDIGVQADA
NAEMRARERRDRLHEALSVNRSRVNQLEKQIAFCEAEMENVQKKLRKLER
DYYQIREQVVSAKAGWCAVMRMVKDNGVERRLHRRELAYMEGGALRSMSD
KALGALRLAVADNEHLRDALRLSEDPKRPERKVQFFIAVYQHLRERIRQD
IIRTDDPVDAIEQMEIELARLTEELTAREQKLAISSKSVANIIRKTIQRE
QNRIRMLNQGLQAVSFGQVRGVRLNVNVRESHAILLDVLSEQQEQHQDLF
NSQRLTFSEAMAKLYQRLNPQVDMGQRLPQTIGEELLDYRNYLELDVEVN
RGSDGWLKAESGALSTGEAIGTGMSILVMVVQSWEEESRRLRGKDISPCR
LLFLDQAARLDAKSIATLFELCERLQMQLIIAAPENISPEKGTTYKLVRK
VFKNHEHVHVVGLRGFG

3D structure

• PDB: 7nz2 Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites.
• Chain: B4
• Resolution: 11.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B4	T69 R73 S195 R199	T68 R72 S194 R198
BS02	dna	B4	L55 T56 S170 I171 T172 Q1327 R1328 L1329 T1332	L54 T55 S169 I170 T171 Q1326 R1327 L1328 T1331
BS03	ATP	B4	R1352 A1364 S1366 T1367 E1369	R1351 A1363 S1365 T1366 E1368
BS04	ATP	B4	N36 G37 A38 G39 K40 S41 T42 G79 K80 R1450	N35 G36 A37 G38 K39 S40 T41 G78 K79 R1449

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0005524 ATP binding

Biological Process

• GO:0006260 DNA replication
• GO:0007059 chromosome segregation
• GO:0030261 chromosome condensation
• GO:0051301 cell division

Cellular Component

• GO:0005737 cytoplasm
• GO:0009295 nucleoid

External links

• PDB: RCSB:7nz2, PDBe:7nz2, PDBj:7nz2
• PDBsum: 7nz2
• PubMed: 34739874
• UniProt: A0A0F7LRY2