Structure of PDB 4v65 Chain B2

Receptor sequence

>4v65B2 (length=178) Species: 562 (Escherichia coli)

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFRK

3D structure

• PDB: 4v65 The Structure of the E. coli Ribosome Before and After Accommodation: Implications for Proofreading
• Chain: B2
• Resolution: 9.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B2	A74 G75 F76 K77 R79	A74 G75 F76 K77 R79
BS02	rna	B2	H4 S23 M25 R29 Q62 L65 I66 K87 V88 T89 R91	H4 S23 M25 R29 Q62 L65 I66 K87 V88 T89 R91
BS03	rna	B2	K32 T34 N36 M37 G38 V39 G40 K68 A69 R70 K71 G75 F76 I78 I84 G85 C86 K87 S120 D122 R124 N126 Y127 S128 G130 R132 R147 R149 D152 T154 T156	K32 T34 N36 M37 G38 V39 G40 K68 A69 R70 K71 G75 F76 I78 I84 G85 C86 K87 S120 D122 R124 N126 Y127 S128 G130 R132 R147 R149 D152 T154 T156

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v65, PDBe:4v65, PDBj:4v65
• PDBsum: 4v65
• UniProt: P62399|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)