Structure of PDB 9fil Chain B

Receptor sequence

>9filB (length=418) Species: 224324 (Aquifex aeolicus VF5)

MRSYPAIPRIYAETTLNMLLKRAKKPRVHSIDEYLKDGGYQALEKALNMS
PEEIIDWVDKSTLRGRGGAGFPTGKKWKFAVQNPGPRYFICNADESEPGT
FKDRIIIERDPHLLIEGIIISSYAIGANEAYIYIRGEYPAGYYILRDAIE
EAKKKGFLGKNILGSGFDLEIYVARGAGAYICGEETALIESLEGKRGHPR
LKPPYPVQKGLWGKPTVVNNVKTIANVPFIISMGWEEYRYIGPSDYAGPK
LFPVSGKVKKPGVYELPMNTTLREVIFKYAGGTLGNKKVKAVFSGALDCF
SSEELDIPMDYSPLGFGGTGTVIVLTEEDDIVEAALKIAEFYEHETCGQC
TPCRVGCYEQANLLEKIYKGEATEQDWEGFDFVNRNIQPTSICGLGAVAG
RLIRQTLEKFPEEWEKYR

3D structure

• PDB: 9fil Structural robustness of the NADH binding site in NADH:ubiquinone oxidoreductase (complex I).
• Chain: B
• Resolution: 2.54 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	B	P199 T346 C347 G348 Q349 C350 C353 I392 C393 G396	P199 T346 C347 G348 Q349 C350 C353 I392 C393 G396
BS02	FNR	B	G65 G67 K76 N92 E95 Y180 G183 E184 V218 N219 N220 T223	G65 G67 K76 N92 E95 Y180 G183 E184 V218 N219 N220 T223
BS03	NAD	B	G67 A69 F71 K76 F79 E185 Y205	G67 A69 F71 K76 F79 E185 Y205

Gene Ontology

Molecular Function

• GO:0046872 metal ion binding
• GO:0048038 quinone binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:1902600 proton transmembrane transport

External links

• PDB: RCSB:9fil, PDBe:9fil, PDBj:9fil
• PDBsum: 9fil
• PubMed: 38960077
• UniProt: O66841|NUOF_AQUAE NADH-quinone oxidoreductase subunit F (Gene Name=nuoF)