Structure of PDB 9bcu Chain B

Receptor sequence

>9bcuB (length=1103) Species: 311400 (Thermococcus kodakarensis)

EPTLTPDELWLVMESYWKEKGFVRQHLDSYNAFIDHGLQDVVNEFGEVVP
DIPNFKVKFGKIRIGQPEFQEAQGQRRPLYPMDARIRNLTYSAPLYLEMI
PVVNGIEQEPVEVRIGELPIMLKSKACRLYGLSDEELIKLGEDPKDPGGY
FIINGSERVIVSIEDLAPNKTLVERDERQNKVVAKVFSYRHGYRALITVE
RKKDGILYVTIPNVPKPVKFVYVMRALGLLTDKEIVEAVSDDPRIQQVLF
DNLEDASDISTQEEALDYIGRLALPGQPKEYRLRRAEHIIDNNLLPHMGV
DPENRRAKAYYLGMMALKVLELSLGLRGEDDKDHYANKRLKLAGDLLKDL
FRVAFGQLVKDMQYQMTKTYQRKGERYTFENIQRFVRNSIRPDVLSERIE
HALATGSWPGGRTGVSQLLDRTNYMSTLSHLRRVTSPLSRDQPHFEARDL
HGTHWGRICPTETPEGPNCGLVKNLALMSQITTGIPEREVREYLMKMGVV
PIEERRPAPGLYRVYLNGVLIGTVEDGRKLVERIRADRRAGKISDVINVA
LYEDEEVKEVYINSDDGRVRRPLIVVENGKPKLTREHVEGIKNGTLTWSD
LIRMGVIEYLDAEEEENAYVATWPWEVTEEHTHLELMPAAILGIPASLVP
YPEHNAAPRNTYGAGMAKQSLGLGWANFRIRVDTRGHLMHYPQVPLVNSR
IMKAVGFEDRPAGQNFVVAVLSYHGYNMEDAVIINKASIERGLARSTFFR
TYEAEEKRYLGGQKDNFEVPSPNIQGYLGEKYYRHLDEDGLIFPESKVEG
KDVLVGRTSPPRFQGRRETSVTVRPSEKGVVDKVIVTETGDGTKLVKVTV
RDLRIPELGDKFASRHGQKGVIGLIVPQEDMPWTESGIVPDLIVNPHGIP
SRMTVGQLIEAIGGKVASLTGRRVDGTAFIGEPEEKLRKELEELGFKHSG
REIMYDGITGRRLEADIFIGVIYYQRLHHMVADKMHARSRGPVQVLTKQP
TEGRAREGGLRFGEMERDVLIGHGAAMLLIERLLEESDKTEVWVCESCGH
LALEDKRRGKVYCPVCGEDERISKVEMSYAFKLLLDELKAMVIRPSLRLK
DRV

3D structure

• PDB: 9bcu Structural basis of archaeal FttA-dependent transcription termination
• Chain: B
• Resolution: 2.2 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R186 S447 Q450	R178 S439 Q442
BS02	dna	B	S164 K224 Y372 K376 R399 H409 T413 L426 T692 R693 R758 R844 R1024 R1031 M1035	S156 K216 Y364 K368 R391 H401 T405 L418 T684 R685 R750 R824 R1004 R1011 M1015
BS03	rna	B	T421 G422 Q425 N476 Q677 K881 K889 P1012 V1013 Q1014	T413 G414 Q417 N468 Q669 K861 K869 P992 V993 Q994
BS04	ZN	B	C1065 C1083 C1086	C1045 C1063 C1066

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005737 cytoplasm

External links

• PDB: RCSB:9bcu, PDBe:9bcu, PDBj:9bcu
• PDBsum: 9bcu
• UniProt: Q5JE32