Structure of PDB 8z9r Chain B

Receptor sequence

>8z9rB (length=676) Species: 126796 (Thogoto virus (isolate SiAr 126))

MNLFTPLSEINPTTTQELLYAYTGPAPVAYGTRTRAVLENIIRPYQYFYK
EPNVQRALDIKTGCKEPEDINVEGPSSGFHTASVLKLADNFFRKYRPAME
KLKYWILVKLPKLKYAELSKGRQTYSFIHKRNLPAPIALEETVEFLEQNL
RRKIGPTLLSYCQAIADVMELDETTYEGARTYTIKFSREELWDQMRTLNT
MWKHLERGRLNRRTIATPSMLIRGFVKIVEDAAKEILENVPTSGVPVGGE
EKLAKLASKQTFHTAVTGELSGDQEKFNECLDPDAMRLMWTVFLRKLGCP
DWIMELFNIPFMVFKSKLADMGEGLVYTKGKLTDRKPLGEMPSEFDDLVR
NVVGNSISCRLGMFMGMYNLTSTLLALISIEREELTGSHVESSDDFIHFF
NCKTHEEMFKQAETLRLTLKLVGINMSPSKCILISPAGIGEFNSKFHHRD
FVGNVATELPALVPNGTNPMTDLAMGLNVIKHSVNTGQMNLCTGALAMRI
FNHAYKYAYMALGVTRRTRFMEENAITPLLTNQGASPVHSFSTMHLDEVA
LRRHLGLLDEETLRRILNPNNPVTQKGDPSMFFRIENKMPQIMEDYSVPS
CFKYTLSRNRHKALLNKEERYQRVTSIINKLFPEVLIQEASAPGTVRESL
KRRLELVVERSDLDEERKKRILSRIF

3D structure

• PDB: 8z9r Cryo-EM structures of Thogoto virus polymerase reveal unique RNA transcription and replication mechanisms among orthomyxoviruses
• Chain: B
• Resolution: 2.58 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	Y30 G31 R35 V354	Y30 G31 R35 V326
BS02	rna	B	H531 Y535 L540 G541 V542 R544	H503 Y507 L512 G513 V514 R516
BS03	rna	B	R122 Q123 N132 M229 I243 A244 R251 K262 P274 G394 M395 N397 T499 M503 N506 H510 K604	R122 Q123 N132 M201 I215 A216 R223 K234 P246 G366 M367 N369 T471 M475 N478 H482 K576
BS04	rna	B	K120 D422 N471 S472 T485 P488 N493	K120 D394 N443 S444 T457 P460 N465

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription

External links

• PDB: RCSB:8z9r, PDBe:8z9r, PDBj:8z9r
• PDBsum: 8z9r
• PubMed: 38816392
• UniProt: O41353|RDRP_THOGV RNA-directed RNA polymerase catalytic subunit (Gene Name=Segment)