Structure of PDB 8z97 Chain B

Receptor sequence

>8z97B (length=670) Species: 126796 (Thogoto virus (isolate SiAr 126))

MNLFTPLSEINPTTTQELLYAYTGPAPVAYGTRTRAVLENIIRPYQYFYK
EPNVQRALDIKTGCKEPEDINVEGPSSGFHTASVLKLADNFFRKYRPAME
KLKYWILVKLPKLKYAELSKGRQTYSFIHKRNLPAPIALEETVEFLEQNL
RRKIGPTLLSYCQAIADVMELDETTYEGARTYTIKFSREELWDQMRTLNT
MWKHLERGRLNRRTIATPSMLIRGFVKIVEDAAKEILENVPTSGVPVGGE
EKLAKLASKQTFHTAVTGELSGDQEKFNECLDPDAMRLMWTVFLRKLGCP
DWIMELFNIPFMVFKSKLADMGEGLVYTKGKLTDRKPLGEMPSEFDDLVR
NVVGNSISCRLGMFMGMYNLTSTLLALISIEREELTGSHVESSDDFIHFF
NCKTHEEMFKQAETLRLTLKLVGINMSPSKCILISPAGIGEFNSKFHHRD
FVGNVATELPALVPNGTNPMTDLAMGLNVIKHSVNTGQMNLCTGALAMRI
FNHAYKYAYMALGVTRRTRFMEENAITPLLTNQGASPVHSFSTMHLDEVA
LRRHLGLLDEETLRRILNPNNPVTQKGDPSMFFRIENKMPQIMEDYSVPS
CFKYTLSRNRKEERYQRVTSIINKLFPEVLIQEASAPGTVRESLKRRLEL
VVERSDLDEERKKRILSRIF

3D structure

• PDB: 8z97 Cryo-EM structures of Thogoto virus polymerase reveal unique RNA transcription and replication mechanisms among orthomyxoviruses
• Chain: B
• Resolution: 2.65 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	Y30 G31 T32 R35 R363	Y30 G31 T32 R35 R335
BS02	rna	B	H531 Y535 L540 G541 V542 R544	H503 Y507 L512 G513 V514 R516
BS03	rna	B	G121 R122 Q123 N132 I243 A244 T245 R251 K262 V273 P274 G277 G394 M503 N506 H510	G121 R122 Q123 N132 I215 A216 T217 R223 K234 V245 P246 G249 G366 M475 N478 H482
BS04	rna	B	Y22 K120 R122 D422 N471 S472 T485 P488	Y22 K120 R122 D394 N443 S444 T457 P460
BS05	V9G	B	L670 I671 Q672 E673	L630 I631 Q632 E633

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription

External links

• PDB: RCSB:8z97, PDBe:8z97, PDBj:8z97
• PDBsum: 8z97
• PubMed: 38816392
• UniProt: O41353|RDRP_THOGV RNA-directed RNA polymerase catalytic subunit (Gene Name=Segment)