Structure of PDB 8z90 Chain B

Receptor sequence

>8z90B (length=710) Species: 126796 (Thogoto virus (isolate SiAr 126))

MNLFTPLSEINPTTTQELLYAYTGPAPVAYGTRTRAVLENIIRPYQYFYK
EPNVQRALDIKTGCKEPEDINVEGPSSGFHTASVLKLADNFFRKYRPAME
KLKYWILVKLPKLKYAELSKGRQTYSFIHKRNLPAPIALEETVEFLEQNL
RRKIGPTLLSYCQAIADVMELDETTYEGARDPRPWDIQLEEIDSDEEDPL
FRQVGREETYTIKFSREELWDQMRTLNTMWKHLERGRLNRRTIATPSMLI
RGFVKIVEDAAKEILENVPTSGVPVGGEEKLAKLASKQTFHTAVTGELSG
DQEKFNECLDPDAMRLMWTVFLRKLGCPDWIMELFNIPFMVFKSKLADMG
EGLVYTKGKLTDRKPLGEMPSEFDDLVRNVVGNSISCRLGMFMGMYNLTS
TLLALISIEREELTGSHVESSDDFIHFFNCKTHEEMFKQAETLRLTLKLV
GINMSPSKCILISPAGIGEFNSKFHHRDFVGNVATELPALVPNGTNPMTD
LAMGLNVIKHSVNTGQMNLCTGALAMRIFNHAYKYAYMALGVTRRTRFME
ENAITPLLTNQGASPVHSFSTMHLDEVALRRHLGLLDEETLRRILNPNNP
VTQKGDPSMFFRIENKMPQIMEDYSVPSCFKYTLSRNRTIQDKPHKALLN
KEERYQRVTSIINKLFPEVLIQEASAPGTVRESLKRRLELVVERSDLDEE
RKKRILSRIF

3D structure

• PDB: 8z90 Cryo-EM structures of Thogoto virus polymerase reveal unique RNA transcription and replication mechanisms among orthomyxoviruses
• Chain: B
• Resolution: 2.87 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	Y30 G31 T32 R35 W185 R363	Y30 G31 T32 R35 W185 R363
BS02	rna	B	R33 R122 Q123 R131 N132 M229 H232 I243 T245 E351 G394 M395 N397 R612 N615 K616 M617 P618 N637 R638 T639 Q641 K643 H645	R33 R122 Q123 R131 N132 M229 H232 I243 T245 E351 G394 M395 N397 R612 N615 K616 M617 P618 N637 R638 T639 Q641 K643 H645
BS03	G2P	B	K231 R241 D301 Q302 K304 F305 N306 G394 D422 K458	K231 R241 D301 Q302 K304 F305 N306 G394 D422 K458

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0001172 RNA-templated transcription

External links

• PDB: RCSB:8z90, PDBe:8z90, PDBj:8z90
• PDBsum: 8z90
• PubMed: 38816392
• UniProt: O41353|RDRP_THOGV RNA-directed RNA polymerase catalytic subunit (Gene Name=Segment)