Structure of PDB 8xrf Chain B

Receptor sequence

>8xrfB (length=774) Species: 10498 (African swine fever virus BA71V)

VDVDKYTRARNAGGKRAQDCMLLAAEGDSALSLLRTGLTLGKSNPSGPSF
DFCGMISLGGVIMNACKKVTNITIMVRNEQLTNNKVLQGIVQVLGLDFNC
HYKTQEERAKLRYGCIVACVDQDLDGCGKILGLLLAYFHLFWPQLIIHGF
VKRLLTPLIRVYEKGKTMPVEFYYEQEFDAWAKKQTSLVNHTVKYYKGLA
AHDTHEVKSMFKHFDNMVYTFTLDDSAKELFHIYFGGESELRKRELCTGV
VPLTETQTQSIHSVRRIPCSLHLQVDTKAYKLDAIERQIPNFLDGMTRAR
RKILAGGVKCFASNNRERKVFQFGGYVADHMFYHHGDMSLNTSIIKAAQY
YPGSSHLYPVFIGIGSFGSRHLGGKDAGSPRYISVQLASEFIKTMFPAED
SWLLPYVFEDGQRAEPEYYVPVLPLAIMEYGANPSEGWKYTTWARQLEDI
LALVRAYVDKDNPKHELLHYAIKHKITILPLRPSNYNFKGHLKRFGQYYY
SYGTYDISEQRNIITITELPLRVPTVAYIESIKKSSNRMTFIEEIIDYSS
SETIEILVKLKPNSLNRIVEEFKETEEQDSIENFLRLRNCLHSHLNFVKP
KGGIIEFNSYYEILYAWLPYRRELYQKRLMREHAVLKLRIIMETAIVRYI
NESAELNLSHYEDEKEASRILSEHGFPPLNHTLIISPEFASIEELNQKAL
QGCYTYILSLQARELLIAAKTRRVEKIKKMQARLDKVEQLLQESPFPGAS
VWLEEIDAVEKAIIKGRNTQWKFH

3D structure

• PDB: 8xrf The crystal structure of AsfvTopII in complex with G-DNA
• Chain: B
• Resolution: 2.94 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	V473 M475 N476 K479 K480 Q498 K547 S657 R660 K661 Q706 R799 Y800 P852 S853 E854 G855 K857 S953 R1004 H1010	V61 M63 N64 K67 K68 Q80 K129 S239 R242 K243 Q288 R381 Y382 P434 S435 E436 G437 K439 S535 R586 H592
BS02	dna	B	E438 V473 D543 R705 R718 Y751 H753 G754 S761 K793 A850 P852 K857	E26 V61 D125 R287 R300 Y333 H335 G336 S343 K375 A432 P434 K439
BS03	dna	B	K417 G439 D440 S441 G472 M756	K5 G27 D28 S29 G60 M338
BS04	MG	B	A1072 N1075	A654 N657
BS05	MG	B	E593 E827	E175 E409
BS06	MG	B	D539 D541 K615	D121 D123 K197

External links

• PDB: RCSB:8xrf, PDBe:8xrf, PDBj:8xrf
• PDBsum: 8xrf
• UniProt: Q00942|TOP2_ASFB7 DNA topoisomerase 2 (Gene Name=TOP)