Structure of PDB 8wr4 Chain B

Receptor sequence

>8wr4B (length=1442) Species: 59732 (Chryseobacterium)

MIKNILGLALGTNSIGWALVKQDFENKQGEILGMGSRIIPMSQDILGDFG
KGNSVSQTAERTKYRSVRRLRERFLLRRERLHRVLYILNFLPEHYASQID
FEKRLGKFKVETEPKLVWKNTDGQFSFLFQNSFNEMLEDFKAAGQELKIP
YDWTIYHLRKKAISQKIEKEELAWILLNFNHKRGYYQLRGEDFEEEKDKT
FVRLKVDRIVDSGENVKGKILYDVYFENGWKYDKQVVKTEDWVDRTKEFI
VSESILKNGETKRTFKAVDSEKDWIAIKTKTEQEIEHSHKTVGTYIYETL
LQNPKQKIKGKLVRTIERKFYKEELRQILEKQKEFHQELQSDDLYNDCIR
ELYRNNEVHQLTLRKKDFVHLFMEDIIFYQRPLRSQKSSVSNCTLEFRKY
KGENGAEHTQYLKAIPKSNPYYQEFRLWQWIFNLNLYTKDNDENVTKVFL
NTTQDFENLFEFLNTRKEVDQKALLKHFKLNEKTHRWNFVEDKKYPCNET
KTMISSRLDKVENISDDFLTRDIEQKIWHIIYSVNDKVEYEKALKSFARK
HHLDESSFFEAFRKFPPFKSEYGSFSEKAIKKLLPLMRLGKYWNYAEIDK
YSRERIQKIITGEYDENIKDKVREKSVHLTIENDFQGLQLWLAQYIVYGR
HSEASMIGKWNSANDLEVFLKDFKQHSLRNPIVEQVITETLRVVKDIWLK
YGNGTKDFFNEIHIELGREMKLPADDRKKLTNQITENENTNLRIKALLAE
MMNDHSVENVRPFSPMQQEILKIYEDGVLKSDIEIEDDILKISKTAQPSS
SDLKRYKLWLEQKYKSPYTGQIIPLNKLFTPEYEIEAIIPQSRYFDDSFS
NKIICESAVNKLKDNYIGLGFIKQFGGTIIELGFGKSVKVFDTEEYEDFV
KKHYANNRGKRNKLLMEDIPEKMIERQLNDTRYISKYISGILSNIVRVED
GSDEGVNSKNIVPGNGKITTQLKQDWGLNDVWNDLILPRFERMNQLTNSK
DFTAWNENHQKFLPTVPIEFSKGFSKKRIDHRHHALDALVIACATTDHVN
LLNNQSAKSDTKRYDLKKKLMKFEKVVYHHTQTGEKIEREIPKQFLKPWE
KFTVDAKHNLESIIVSFKQNLRVINKATNYYEKYVEKDGTKNKERVEQAG
TNWAIRKPMHKDTVSGKVDLPWVKVPKGKILTATRKSLDSSFDLKSIGSI
TDTGIQKILKNYLAFKDGNPELAFSPEGIDDLNKNIEKYNDGKPHQPINK
VRVFELGSKFQVGQTGNKKGKYVEAAKGTNLFFAVYEDEKGKRSYETIPL
NEVIERQKQGLTSVPLENEKGSRLLFDLSPNDLVYVPEIDENIDSNFVFS
NLNKEKISRIYKVEKTSGTECYFVRQDIAYLIKQYDAKTKIGELESQNKL
QVTMTDDRIRITDTCVKINCDRLGNINFITKEKIKQIFNEFR

3D structure

• PDB: 8wr4 Pro-CRISPR PcrIIC1-associated Cas9 system for enhanced bacterial immunity.
• Chain: B
• Resolution: 3.07 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	K257 K1277	K257 K1277
BS02	rna	B	S54 T58 R61 T62 Y64 R65 R68 R69 L70 R71 R73 L76 R78 R80 K107 K115 W118 F125 K148 Y151 D152 W153 W174 N178 H181 K182 R183 G184 K307 I308 K309 G310 R314 T315 Y353 N356 D375 Y379 Q380 R381 L383 R384 S385 Q386 S388 R398 Y400 E403 Q410 K417 N488 F489 H529 I530 Y532 S533 E539 K542 A543 K550 E571 Y572 E577 K578 Q675 H676 R679 R692 P723 T931 R932 Y933 R1122 N1125 K1126 N1129 Y1130 Y1131 E1132 K1133 K1143 R1145 Q1148 N1152 W1153 I1155 R1156 P1158 M1159 H1160 V1164 D1169 R1185 T1201 D1202 T1203 G1204 K1243 P1244 H1245 Q1246 P1247 N1249 K1250 R1252 V1262 T1265 K1268 G1270 K1271 K1308	S54 T58 R61 T62 Y64 R65 R68 R69 L70 R71 R73 L76 R78 R80 K107 K115 W118 F125 K148 Y151 D152 W153 W174 N178 H181 K182 R183 G184 K307 I308 K309 G310 R314 T315 Y353 N356 D375 Y379 Q380 R381 L383 R384 S385 Q386 S388 R398 Y400 E403 Q410 K417 N488 F489 H529 I530 Y532 S533 E539 K542 A543 K550 E571 Y572 E577 K578 Q675 H676 R679 R692 P723 T931 R932 Y933 R1122 N1125 K1126 N1129 Y1130 Y1131 E1132 K1133 K1143 R1145 Q1148 N1152 W1153 I1155 R1156 P1158 M1159 H1160 V1164 D1169 R1185 T1201 D1202 T1203 G1204 K1243 P1244 H1245 Q1246 P1247 N1249 K1250 R1252 V1262 T1265 K1268 G1270 K1271 K1308

External links

• PDB: RCSB:8wr4, PDBe:8wr4, PDBj:8wr4
• PDBsum: 8wr4
• PubMed: 38811729