Structure of PDB 8wq9 Chain B

Receptor sequence

>8wq9B (length=478) Species: 208964 (Pseudomonas aeruginosa PAO1)

SLLIRGATVVTHEESYRADVLCANGLIQAIGENLETPSGCDVLDGGGQYL
MPGGIDPHTHMQLPFMGTVASEDFFSGTAAGLAGGTTSIIDFVIPNPRQS
LLEAFHTWRGWAQKSAADYGFHVAITWWSDEVAREMGELVAQHGVNSFKH
FMAYKNAIMAADDTLVASFERCLELGAVPTVHAENGELVFHLQQKLLAQG
LTGPEAHPLSRPPQVEGEAASRAIRIAETLGTPLYLVHISSREALDEIAY
ARAKGQPVYGEVLAGHLLLDDSVYRHPDWATAAGYVMSPPFRPVEHQEAL
WRGLQSGNLHTTATDHCCFCAEQKAMGRDDFSKIPNGTAGIEDRMALLWD
AGVNSGRLSMHEFVALTSTNTAKIFNLFPRKGAIRVGADADLVLWDPQGS
RTLSAATHHQRVDFNIFEGRTVRGIPSHTISQGKLLWAAGDLRAEPGAGR
YVERPAYPSVYEVLGRRAERQRPVAVER

3D structure

• PDB: 8wq9 The complexed crystal structure of dihydropyrimidinase reveals a potential interactive link with the neurotransmitter gamma-aminobutyric acid (GABA).
• Chain: B
• Resolution: 1.97 Å

Enzymatic activity

• Enzyme Commision number: 3.5.2.2: dihydropyrimidinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ABU	B	F66 F320 I335 N337	F65 F319 I334 N336
BS02	ABU	B	H61 K150 F152	H60 K149 F151
BS03	ZN	B	K150 H183 H239	K149 H182 H238
BS04	ZN	B	H59 H61 K150 D316	H58 H60 K149 D315

Gene Ontology

Molecular Function

• GO:0004157 dihydropyrimidinase activity
• GO:0016787 hydrolase activity
• GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
• GO:0016812 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
• GO:0046872 metal ion binding

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8wq9, PDBe:8wq9, PDBj:8wq9
• PDBsum: 8wq9
• PubMed: 38056157
• UniProt: Q9I676|HYDA_PSEAE D-hydantoinase/dihydropyrimidinase (Gene Name=dht)