Structure of PDB 8wej Chain B

Receptor sequence

>8wejB (length=569) Species: 9606 (Homo sapiens)

GNWAVNEGLSIFVILVWLGLNVFLFVWYYRVYDIPPKFFYTRKLLGSALA
LARAPAACLNFNCMLILLPVCRNLLSFLRGSSACCSTRVRRQLDRNLTFH
KMVAWMIALHSAIHTIAHLFNVEWCVNARVNNSDPYSVALSELGDRQNES
YLNFARKRIKNPEGGLYLAVTLLAGITGVVITLCLILIITSSTKTIRRSY
FEVFWYTHHLFVIFFIGLAIHGAERIVRGQTAESLAVHNITVCEQKISEW
GKIKECPIPQFAGNPPMTWKWIVGPMFLYLCERLVRFWRSQQKVVITKVV
THPFKTIELQMKKKGFKMEVGQYIFVKCPKVSKLEWHPFTLTSAPEEDFF
SIHIRIVGDWTEGLFNACGCDKQEFQDAWKLPKIAVDGPFGTASEDVFSY
EVVMLVGAGIGVTPFASILKSVWYKYCNNATNLKLKKIYFYWLCRDTHAF
EWFADLLQLLESQMQERNNAGFLSYNIYLTGWDESQANHFAVHHDEEKDV
ITGLKQKTLYGRPNWDNEFKTIASQHPNTRIGVFLCGPEALAETLSKQSI
SNSESGPRGVHFIFNKENF

3D structure

• PDB: 8wej Activation mechanism of phagocyte NADPH oxidase by cytosolic factors
• Chain: B
• Resolution: 2.79 Å

Enzymatic activity

• Enzyme Commision number: 1.-.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	B	A57 L60 N61 S112 H115 T116 H119 A175 G179 I182 T183 F215 L219 H222 R226 I227	A56 L59 N60 S111 H114 T115 H118 A174 G178 I181 T182 F214 L218 H221 R225 I226
BS02	HEM	B	V71 R73 H101 K102 A105 I189 S193 R198 F205 W206 H209 F212 F215 F216 Y280	V70 R72 H100 K101 A104 I188 S192 R197 F204 W205 H208 F211 F214 F215 Y279
BS03	NDP	B	A409 G410 I411 L444 C445 R446 T481 R513 G538 P539 L542 E568 F570	A408 G409 I410 L443 C444 R445 T480 R512 G537 P538 L541 E567 F569
BS04	FDA	B	F202 Y324 H338 P339 F340 T341 H354 I355 R356 G359 D360 W361 T362 I411 T414 F570	F201 Y323 H337 P338 F339 T340 H353 I354 R355 G358 D359 W360 T361 I410 T413 F569

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0009055 electron transfer activity
• GO:0016175 superoxide-generating NAD(P)H oxidase activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0046982 protein heterodimerization activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050664 oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor

Biological Process

• GO:0006801 superoxide metabolic process
• GO:0006952 defense response
• GO:0006954 inflammatory response
• GO:0007584 response to nutrient
• GO:0009410 response to xenobiotic stimulus
• GO:0032760 positive regulation of tumor necrosis factor production
• GO:0034220 monoatomic ion transmembrane transport
• GO:0042554 superoxide anion generation
• GO:0045087 innate immune response
• GO:0045730 respiratory burst
• GO:0045766 positive regulation of angiogenesis
• GO:0050665 hydrogen peroxide biosynthetic process
• GO:0071276 cellular response to cadmium ion
• GO:0071361 cellular response to ethanol
• GO:0071456 cellular response to hypoxia
• GO:0097411 hypoxia-inducible factor-1alpha signaling pathway
• GO:1904044 response to aldosterone
• GO:1904845 cellular response to L-glutamine
• GO:1990776 response to angiotensin

Cellular Component

• GO:0005635 nuclear envelope
• GO:0005737 cytoplasm
• GO:0005789 endoplasmic reticulum membrane
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0030425 dendrite
• GO:0030670 phagocytic vesicle membrane
• GO:0034702 monoatomic ion channel complex
• GO:0035579 specific granule membrane
• GO:0043020 NADPH oxidase complex
• GO:0043025 neuronal cell body
• GO:0045335 phagocytic vesicle
• GO:0070821 tertiary granule membrane
• GO:0097038 perinuclear endoplasmic reticulum

External links

• PDB: RCSB:8wej, PDBe:8wej, PDBj:8wej
• PDBsum: 8wej
• PubMed: 38355798
• UniProt: P04839|CY24B_HUMAN Cytochrome b-245 heavy chain (Gene Name=CYBB)