Structure of PDB 8wa1 Chain B

Receptor sequence
>8wa1B (length=998) Species: 4097 (Nicotiana tabacum) [Search protein sequence]
NEGISTIPGFNQIQFEGFCRFIDQGLTEELYKFPKIEDTDQEIEFQLFVE
TYQLVEPLIKERDAVYESLTYSSELYVSAGLIWKNSRDMQEQTIFIGNIP
LMNSLGTSIVNGIYRIVINQILQSPGIYYRSELDHNGISVYTGTIISDWG
GRSELEIDRKARIWARVSRKQKISILVLSSAMGLNLREILENVCYPEIFL
SFLFFQQRCELGRIGRRNMNRRLNLDIPQNNTFLLPRDILAAADHLIGLK
FGMGALDDMNHLKNKRIRSVADLLQDQFGLALVRLENVVRGTICGAIRHK
LIPTPQNLVTSTPLTTTYESFFGLHPLSQVLDRTNPLTQIVHGRKLSYLG
PGGLTGRTASFRIRDIHPSHYGRICPIDTSEGINVGLIGSLAIHARIGHW
GSLESPFYEISERSTGVRMLYLSPGRDEYYMVAAGNSLALNQDIQEEQVV
PARYRQEFLTIAWEQVHLRSIFPFQYFSIGASLIPFIEHNDANRALMSSN
MQRQAVPLSRSEKCIVGTGLERQAALDSGALAIAEREGRVVYTNTDKILL
AGNGDILSIPLVIYQRSNKNTCMHQKLQVPRGKCIKKGQILADGAATVGG
ELALGKNVLVAYMPWEGYNSEDAVLISERLVYEDIYTSFHIRKYEIQTHV
TSQGPEKVTNEIPHLEAHLLRNLDKNGIVMLGSWVETGDILVGKLTPQSK
ETCLKLPIGGRGRVIDVRWIQKRGGSSYNPETIRVYILQKREIKVGDKVA
GRHGNKGIISKILPRQDMPYLQDGRSVDMVFNPLGVPSRMNVGQIFECSL
GLAGSLLDRHYRIAPFDERYEQEASRKLVFSELYEASKQTANPWVFEPEY
PGKSRIFDGRTGNPFEQPVIIGKPYILKLIHQVDDKIHGRSSGHYALVTQ
QPLRGRAKQGGQRVGEMEVWALEGFGVAHILQEMLTYKSDHIRARQEVLG
TTIIGGTIPNPEDAPESFRLLVRELRSLALELNHFLVSEKNFQINRKE
3D structure
PDB8wa1 Cryo-EM structures of the plant plastid-encoded RNA polymerase.
ChainB
Resolution2.8 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B R404 T405 R357 T358
BS02 dna B R120 G370 R404 G976 R977 M988 R115 G323 R357 G905 R906 M917
BS03 rna B Q376 R550 K827 H952 A967 L968 Q329 R503 K756 H881 A896 L897
BS04 ZN B E535 H536 D888 E889 S896 E488 H489 D817 E818 S825
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0009507 chloroplast

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8wa1, PDBe:8wa1, PDBj:8wa1
PDBsum8wa1
PubMed38428393
UniProtP06271|RPOB_TOBAC DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)

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