Structure of PDB 8wa0 Chain B

Receptor sequence

>8wa0B (length=973) Species: 4097 (Nicotiana tabacum)

NEGISTIPGFNQIQFEGFCRFIDQGLTEELYKFPKIEDTDQEIEFQLFVE
TYQLVEPLIKERDAVYESLTYSSELYVSAGLIWKNSRDMQEQTIFIGNIP
LMNSLGTSIVNGIYRIVINQILQSPGIYYRSELDHNGISVYTGTIISDWG
GRSELEIDRKARIWARVSRKQKISILVLSSAMGLNLREILENVCYPEIFL
SFLFFQQRCELGRIGRRNMNRRLNLDIPQNNTFLLPRDILAAADHLIGLK
FGMGALDDMNHLKNKRIRSVADLLQDQFGLALVRLENVVRGTICGAIRHK
LIPTPQNLVTSTPLTTTYESFFGLHPLSQVLDRTNPLTQIVHGRKLSYLG
PGGLTGRTASFRIRDIHPSHYGRICPIDTSEGINVGLIGSLAIHARIGHW
GSLESPFYEISERSTGVRMLYLSPGRDEYYMVAAGNSLALNQDIQEEQVV
PARYRQEFLTIAWEQVHLRSIFPFQYFSIGASLIPFIEHNDANRALMSSN
MQRQAVPLSRSEKCIVGTGLERQAALDSGALAIAEREGRVVYTNTDKILL
AGNGDILSIPLVIYQRSNKNTCMHQKLQVPRGKCIKKGQILADGAATVGG
ELALGKNVLVAYMPWEGYNSEDAVLISERLVYEDIYTSFHIRKYEIQTAH
LLRNLDKNGIVMLGSWVETGDILVGETCLKLPIGGRGRVIDVRWIQKRGG
SSYNPETIRVYILQKREIKVGDKVAGRHGNKGIISKILPRQDMPYLQDGR
SVDMVFNPLGVPSRMNVGQIFECSLGLAGSLLDRHYRIAPFDERYEQEAS
RKLVFSELYEASKQTANPWVFEPEYPGKSRIFDGRTGNPFEQPVIIGKPY
ILKLIHQVDDKIHGRSSGHYALVTQQPLRGRAKQGGQRVGEMEVWALEGF
GVAHILQEMLTYKSDHIRARQEVLGTTIIGGTIPNPEDAPESFRLLVREL
RSLALELNHFLVSEKNFQINRKE

3D structure

• PDB: 8wa0 Cryo-EM structures of the plant plastid-encoded RNA polymerase.
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	R120 G370 R975 R977 R984	R115 G323 R879 R881 R888
BS02	rna	B	Q551 H952	Q504 H856
BS03	ZN	B	E535 H536 D888 E889 S896	E488 H489 D792 E793 S800

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0009507 chloroplast

External links

• PDB: RCSB:8wa0, PDBe:8wa0, PDBj:8wa0
• PDBsum: 8wa0
• PubMed: 38428393
• UniProt: P06271|RPOB_TOBAC DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)