Structure of PDB 8vxy Chain B

Receptor sequence

>8vxyB (length=1161) Species: 562 (Escherichia coli)

MPATADEIIEAIKEASAVGFRGRLIARGQARSVIWRDGDLPPDAPEFSAL
LSQDLQGYAYALIDLGLRLRELNGDDAYARIAFEQAGTALESAIAKGKRD
SRDTDFHFVMAAASYHLAHLSARAYSLLAMVGQDDNFSPIERALTQLIRR
DLRTLRDNALGFRLRGDGSDVKITEILQARLNLPQDENGDSESEEDILFD
GLDLALTDAYMSAISLYLLAVERGESRLLSRAIEKLRISLSICAQFNMLP
QWWLNFITIHLLSDLWSDTFHERLPLVPVGGDAAEWPALRELFIALLQRR
PRAEIDLWPSQREAAGRSVNDNDDLVVSLPTSAGKTRIAELCILRCLAGG
KRVVFITPLRALSAQTEATLSRTFGPLGKTISMLYGSIGVSGMDEDAIRQ
RDIVVATPEKLDFALRNDPSIINDVGLFIFDEGHMIGADEREVRYEVQIQ
RLLRRQDADTRRIVCLSAILPDGEQLDDFAGWLRRDKPGGPIKNNWRPTR
LQFGEVIWSAPAGRLNLSVGYEAAWVSRFIVSRQPPFPSDNKELCLATAW
RLIEDGQTVLIYCPLRRSVEPFAETIVDLHQRGLLPSLFDAAPDILDTAI
SLGEEWLGAHSPILACLRLGVALHHGALPTAYRKEIERLLRDGVLKVTIS
SPTLAQGLNLSATAIVMHSLHRNRELIKVSEFRNVIGRAGRAYVDVEGLV
IYPIFDKVNKRQTNWHTLTSDTGAREMESGLIQLVCVLLIRMHTRLGGDL
KALTEYVTNNAVAWEFPEIMTESPQERDIAQAIWEKQLSTLDTAILSLLG
ENDIPDDQIETALDDILQSSLWQRSLQRYRDENERILLKSGLLSRSRYIW
QRSTAAGRRGYFLSGVGLTTGLRLDAIAAKANQLLIDANAAIMGGDAEEA
IAAITALAEEVFTFYPFIPDPLPGDWRGILRSWLLGEPMTNVANTQASET
LQFVENGLVYRLPWAMEAIRVRATANGDLIGDTDTTLDDYELGFAVAAVE
TGTLSRSSSLLIQAGFSSRLAAIKVVTDTTADFQSGQELRRWLNSEEVIS
HTDNHDWPTPETRVMWLEFLGSLSPKGSQVWSRHRYNGMVDWRDTPAVIG
TPLQLYTVDGIHHVLADDGTPLGSINGRINTNRRGLLRVEVDDENGRAMF
DYLGPDDFIST

3D structure

• PDB: 8vxy Hachiman is a genome integrity sensor
• Chain: B
• Resolution: 3.19 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	P535 F550 S552 Q594 R595 H684 R685 R687 E688 K723 N727	P535 F537 S539 Q581 R582 H671 R672 R674 E675 K710 N714
BS02	dna	B	P536 D553 N554 K555 S581 H681 F718 D719 K720 K723 R724	P536 D540 N541 K542 S568 H668 F705 D706 K707 K710 R711
BS03	ATP	B	E304 D306 W308 L329 T331 S332 A333 G334 K335 T336 R337 Y706	E304 D306 W308 L329 T331 S332 A333 G334 K335 T336 R337 Y693

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity

External links

• PDB: RCSB:8vxy, PDBe:8vxy, PDBj:8vxy
• PDBsum: 8vxy
• PubMed: 38464307
• UniProt: A0A426EXV0