Structure of PDB 8vlg Chain B

Receptor sequence

>8vlgB (length=540) Species: 9606 (Homo sapiens)

AELKMDQALLLIHNELLWTNLTVYWKSECCYHCLFQVLVNVPQSPKAGKP
SAAAASVSTQHGSILQLNDTLEEKEVCRLEYRFGEFGNYSLLVKNIIACD
LAVNEDPVDSNLPVSIAFLIGLAVIIVISFLRLLLSLDDFNNWISKAIPP
RLRSVDTFRGIALILMVFVNYGGGKYWYFKHASWNGLTVADLVFPWFVFI
MGSSIFLSMTSILQRGCSKFRLLGKIAWRSFLLICIGIIIVNPNYCLGPL
SWDKVRIPGVLQRLGVTYFVVAVLELLFAKPVPSCLSLRDITSSWPQWLL
ILVLEGLWLGLTFLLPVPGCPTGYLGPGGIGDFGKYPNCTGGAAGYIDRL
LLGDDHLYQHPSSAVLYHTEVAYDPEGILGTINSIVMAFLGVQAGKILLY
YKARTKDILIRFTAWCCILGLISVALTKVSENEGFIPVNKNLWSLSYVTT
LSSFAFFILLVLYPVVDVKGLWTGTPFFYPGMNSILVYVGHEVFENYFPF
QWKLKDNQSHKEHLTQNIVATALWVLIAYILYRKKIFWKI

3D structure

• PDB: 8vlg Structural and mechanistic insights into a lysosomal membrane enzyme HGSNAT involved in Sanfilippo syndrome
• Chain: B
• Resolution: 3.15 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ACO	B	R267 L268 D272 R275 M282 N286 F310 F313 I316 M317 S320 L323 S324 R345 V376 L380 S607 I608 Y611 K662	R151 L152 D156 R159 M166 N170 F194 F197 I200 M201 S204 L207 S208 R229 V260 L264 S484 I485 Y488 K539
BS02	A1ACY	B	N286 H297 R372 V376 E499 K563 E615 E618	N170 H181 R256 V260 E376 K440 E492 E495

Gene Ontology

Molecular Function

• GO:0015019 heparan-alpha-glucosaminide N-acetyltransferase activity
• GO:0016746 acyltransferase activity

Biological Process

• GO:0007041 lysosomal transport
• GO:0030200 heparan sulfate proteoglycan catabolic process
• GO:0051259 protein complex oligomerization

Cellular Component

• GO:0005764 lysosome
• GO:0005765 lysosomal membrane
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0035579 specific granule membrane
• GO:0043202 lysosomal lumen
• GO:0070821 tertiary granule membrane

External links

• PDB: RCSB:8vlg, PDBe:8vlg, PDBj:8vlg
• PDBsum: 8vlg
• UniProt: Q68CP4|HGNAT_HUMAN Heparan-alpha-glucosaminide N-acetyltransferase (Gene Name=HGSNAT)