Structure of PDB 8uis Chain B

Receptor sequence
>8uisB (length=1136) Species: 9823 (Sus scrofa) [Search protein sequence]
DEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPP
IDLQAPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLY
VDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELN
ECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCL
ENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRA
LGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRG
AKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLA
ALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDR
GKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTF
ASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKN
LALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVG
IHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPL
LIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVML
AMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQ
SAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAG
INSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQ
EEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPT
KRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASR
HGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGK
VSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKIT
SQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGE
MERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECR
GCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV
3D structure
PDB8uis Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing.
ChainB
Resolution3.23 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna B Q468 E516 Q731 R841 H842 K942 H1053 Q443 E491 Q706 R816 H817 K904 H1015
BS02 dna B S190 T746 R812 R1080 L1084 R1085 S165 T721 R787 R1042 L1046 R1047
BS03 ZN B C1122 C1140 C1084 C1102
Gene Ontology
Molecular Function
GO:0001055 RNA polymerase II activity
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0003968 RNA-dependent RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0032549 ribonucleoside binding
GO:0034062 5'-3' RNA polymerase activity
GO:0046872 metal ion binding
Biological Process
GO:0001172 RNA-templated transcription
GO:0006351 DNA-templated transcription
GO:0006366 transcription by RNA polymerase II
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0000781 chromosome, telomeric region
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005665 RNA polymerase II, core complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8uis, PDBe:8uis, PDBj:8uis
PDBsum8uis
PubMed38401543
UniProtI3LGP4

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