Structure of PDB 8ugs Chain B

Receptor sequence
>8ugsB (length=816) Species: 9913 (Bos taurus) [Search protein sequence]
QVHRFLDQNPGFADQYFGRKLSPEDVANACEDGCPEGCTSFRELCQVEES
AALFELVQDMQENVNMERVVFKILRRLCSILHADRCSLFMYRQRNGVAEL
ATRLFSVQPDSVLEDCLVPPDSEIVFPLDIGVVGHVAQTKKMVNVQDVME
CPHFSSFADELTDYVTRNILATPIMNGKDVVAVIMAVNKLDGPCFTSEDE
DVFLKYLNFGTLNLKIYHLSYLHNCETRRGQVLLWSANKVFEELTDIERQ
FHKAFYTVRAYLNCDRYSVGLLDMTKEKEFFDVWPVLMGEAQAYSGPRTP
DGREILFYKVIDYILHGKEDIKVIPSPPADHWALASGLPTYVAESGFICN
IMNAPADEMFNFQEGPLDDSGWIVKNVLSMPIVNKKEEIVGVATFYNRKD
GKPFDEQDEVLMESLTQFLGWSVLNTDTYDKMNKLENRKDIAQDMVLYHV
RCDREEIQLILPTRERLGKEPADCEEDELGKILKEVLPGPAKFDIYEFHF
SDLECTELELVKCGIQMYYELGVVRKFQIPQEVLVRFLFSVSKGYRRITY
HNWRHGFNVAQTMFTLLMTGKLKSYYTDLEAFAMVTAGLCHDIDHRGTNN
LYQMKSQNPLAKLHGSSILERHHLEFGKFLLSEETLNIYQNLNRRQHEHV
IHLMDIAIIATDLALYFKKRTMFQKIVDESKNYEDRKSWVEYLSLETTRK
EIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLDQQPI
PMMDRNKAAELPKLQVGFIDFVCTFVYKEFSRFHEEILPMFDRLQNNRKE
WKALADEYEAKVKALE
3D structure
PDB8ugs Probing the mechanism by which the retinal G protein transducin activates its biological effector PDE6.
ChainB
Resolution3.2 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.4.35: 3',5'-cyclic-GMP phosphodiesterase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PCG B R91 S93 F111 S112 F132 G137 V138 V139 A164 Y170 T172 M191 R85 S87 F105 S106 F126 G131 V132 V133 A158 Y164 T166 M185
BS02 ZN B H561 H597 D598 H555 H591 D592
BS03 PCG B H557 H561 D598 L669 D718 L719 F774 H551 H555 D592 L663 D712 L713 F768
Gene Ontology
Molecular Function
GO:0004114 3',5'-cyclic-nucleotide phosphodiesterase activity
GO:0004115 3',5'-cyclic-AMP phosphodiesterase activity
GO:0004117 calmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity
GO:0008081 phosphoric diester hydrolase activity
GO:0016787 hydrolase activity
GO:0046872 metal ion binding
GO:0047555 3',5'-cyclic-GMP phosphodiesterase activity
GO:0048101 calmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity
Biological Process
GO:0007165 signal transduction
GO:0007601 visual perception
GO:0019933 cAMP-mediated signaling
GO:0043153 entrainment of circadian clock by photoperiod
GO:0060041 retina development in camera-type eye
Cellular Component
GO:0001750 photoreceptor outer segment
GO:0016020 membrane
GO:0042622 photoreceptor outer segment membrane
GO:0042995 cell projection
GO:0097381 photoreceptor disc membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8ugs, PDBe:8ugs, PDBj:8ugs
PDBsum8ugs
PubMed38159849
UniProtP23439|PDE6B_BOVIN Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta (Gene Name=PDE6B)

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