Structure of PDB 8tbt Chain B

Receptor sequence

>8tbtB (length=518) Species: 9606 (Homo sapiens) [Search protein sequence]

QQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKE
MIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALD
TKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPN
IVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPG
AQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGH
GIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMM
IGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSG
ETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTA
IGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQV
HLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVT
GWRPGSGYTNIMRVLSIS

3D structure

PDB

8tbt Structure-Based Design of AG-946, a Pyruvate Kinase Activator.

Chain

Resolution

2.34 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.1.40: pyruvate kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	I07	B	F69 D397 Q436 L437 E440	F13 D341 Q380 L381 E384
BS02	FBP	B	L474 T475 T476 T477 S480 R532 G557 R559 G561 S562 G563 Y564 T565	L418 T419 T420 T421 S424 R476 G501 R503 G505 S506 G507 Y508 T509
BS03	PYR	B	K313 E315 A336 G338 D339 T371	K257 E259 A280 G282 D283 T315
BS04	MN	B	E315 D339	E259 D283

Gene Ontology

	Molecular Function
GO:0000287	magnesium ion binding
GO:0003824	catalytic activity
GO:0004743	pyruvate kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0030955	potassium ion binding
GO:0046872	metal ion binding
GO:0048029	monosaccharide binding

	Biological Process
GO:0001666	response to hypoxia
GO:0006096	glycolytic process
GO:0007584	response to nutrient
GO:0009749	response to glucose
GO:0010038	response to metal ion
GO:0016310	phosphorylation
GO:0032869	cellular response to insulin stimulus
GO:0033198	response to ATP
GO:0042866	pyruvate biosynthetic process
GO:0051591	response to cAMP
GO:0071872	cellular response to epinephrine stimulus

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0070062	extracellular exosome

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8tbt, PDBe:8tbt, PDBj:8tbt
PDBsum	8tbt
PubMed	38109501
UniProt	P30613\|KPYR_HUMAN Pyruvate kinase PKLR (Gene Name=PKLR)

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