Structure of PDB 8t70 Chain B

Receptor sequence

>8t70B (length=486) Species: 5207 (Cryptococcus neoformans)

ATEFTPSVYSLVSKPLPSNSRPSATLDEQAETEDLISQLFDLTADPNALV
SEHGKRYSGLRKQEHTQFLASSFFQLPGKFVSLDASRPWLVFWTVHSLDL
LGVALDQGTKDRVVSTLLHFLSPKGGFGGGPANSQIPHLLPTYASVCSLA
IAGNDSSTGGWKDLAAARQSIYEFFMRCKRPDGGFVVCEGGEVDVRGTYC
LLVVATLLDIITPELLHNVDKFVSACQTYEGGFACASFPFPSEPSCRVSM
AEAHGGYTSCSLNSHFLLTSVPLPSFPLSIDANAALRWTVLQQGEPIEGG
GFRGRTNKLVDGCYSWWVGGGAPVAEELVRREKSRKVIPPIFNRVALQEF
TLVAAQQDPGSTGGLRDKPGKRPDQYHTCNNLSGLSIAQHKMSHSPSTVS
SNRLKFDASKGLPAVKPVAPGGGWKNEDERQNARREIWANALGWIEEEGG
EIIVGGKDNRINTTTPVFNILGLRLKPFINYFYCQE

3D structure

• PDB: 8t70 Cryptococcus neoformans protein farnesyltransferase in complex with FPTII and TKCMIIM peptide
• Chain: B
• Resolution: 1.892 Å

Enzymatic activity

• Enzyme Commision number: 2.5.1.58: protein farnesyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	A86 S87 W90 W94 H139 L141 P142 R197 D407 Q408 Y409	A85 S86 W89 W93 H138 L140 P141 R196 D374 Q375 Y376
BS02	ZN	B	D323 C325 H410	D311 C313 H377
BS03	3FX	B	G489 K490 D491	G456 K457 D458
BS04	3FX	B	R62 Q64	R61 Q63
BS05	3FX	B	P124 K125 A133 S135 Q136	P123 K124 A132 S134 Q135
BS06	FII	B	R197 Y200 H266 G268 Y269 C272 R317 K320 Y326 W329	R196 Y199 H254 G256 Y257 C260 R305 K308 Y314 W317
BS07	BT6	B	C325 Y326 H410	C313 Y314 H377

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004659 prenyltransferase activity
• GO:0004660 protein farnesyltransferase activity
• GO:0008270 zinc ion binding
• GO:0008318 protein prenyltransferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0018343 protein farnesylation
• GO:0097354 prenylation

Cellular Component

• GO:0005965 protein farnesyltransferase complex

External links

• PDB: RCSB:8t70, PDBe:8t70, PDBj:8t70
• PDBsum: 8t70
• UniProt: T2BPA1