Structure of PDB 8syh Chain B

Receptor sequence

>8syhB (length=362) Species: 262724 (Thermus thermophilus HB27)

WVKILSVVGARPQFIKAAAVSRVLRASPGVREVLVHTGQHYDDNMSQVFF
EELEIPDPDYHLGIGGGTHGQNTGRMLEAIEGVLLKEKPDWVLVYGNTDS
TLAGALAAVKLHIPVAHVEAGLRSFNRRMPEEINRILTDHASDLLFAPTE
TAVQNLLREGIPENRIHLVGDVMYDAALHYGAKAERKSRILERLGLQAKG
YVLATIHRAENTDDQERLRVILEALAEVHQEVPVVFPVHPRTRKRAEAFG
LGSYLEKVVALEPVGYLDMVMLEKNARLIVTDSGGVQKEAYFYRVPCVTV
REETEWVELLKAEWNYLAAPQNAKDLALTILHRMRTKGVEIDLYGDGRAS
QKISDFLRKVGI

3D structure

• PDB: 8syh Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis.
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.14: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MJL	B	R12 P13 Q14 I16 K17 F50 G97 N98 E120 E132 R136 H208 R209 V265 Y267 M270 S284 G285 G286 E290 E306 W307	R11 P12 Q13 I15 K16 F49 G96 N97 E119 E131 R135 H207 R208 V264 Y266 M269 S283 G284 G285 E289 E305 W306
BS02	UDP	B	Q40 H41 Y42 D43 H240 P241 R242 K245	Q39 H40 Y41 D42 H239 P240 R241 K244

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0008761 UDP-N-acetylglucosamine 2-epimerase activity
• GO:0016853 isomerase activity

External links

• PDB: RCSB:8syh, PDBe:8syh, PDBj:8syh
• PDBsum: 8syh
• PubMed: 37660908
• UniProt: Q72KY0