Structure of PDB 8syd Chain B

Receptor sequence

>8sydB (length=371) Species: 262724 (Thermus thermophilus HB27)

NLYFQGHMWVKILSVVGARPQFIKAAAVSRVLRASPGVREVLVHTGQHYD
DNMSQVFFEELEIPDPDYHLGIGGGTHGQNTGRMLEAIEGVLLKEKPDWV
LVYGNTDSTLAGALAAVKLHIPVAHVEAGLRSFNRRMPEEINRILTDHAS
DLLFAPTETAVQNLLREGIPENRIHLVGDVMYDAALHYGAKAERKSRILE
RLGLQAKGYVLATIHRAENTDDQERLRVILEALAEVHQEVPVVFPVHPRT
RKRAEAFGLGSYLEKVVALEPVGYLDMVMLEKNARLIVTDSGGVQKEAYF
YRVPCVTVREETEWVELLKAEWNYLAAPQNAKDLALTILHRMRTKGVEID
LYGDGRASQKISDFLRKVGIR

3D structure

• PDB: 8syd Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.14: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MJL	B	R12 P13 Q14 I16 K17 F50 G97 N98 E120 E132 R136 H208 R209 V265 M270 S284 G285 G286 E290 E306 W307	R19 P20 Q21 I23 K24 F57 G104 N105 E127 E139 R143 H215 R216 V272 M277 S291 G292 G293 E297 E313 W314
BS02	UD1	B	P13 Q40 H41 Y42 D43 G67 T99 D100 E132 H208 R209 H240 P241 R242 K245	P20 Q47 H48 Y49 D50 G74 T106 D107 E139 H215 R216 H247 P248 R249 K252

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0008761 UDP-N-acetylglucosamine 2-epimerase activity
• GO:0016853 isomerase activity

External links

• PDB: RCSB:8syd, PDBe:8syd, PDBj:8syd
• PDBsum: 8syd
• PubMed: 37660908
• UniProt: Q72KY0