Structure of PDB 8sy9 Chain B

Receptor sequence

>8sy9B (length=361) Species: 262724 (Thermus thermophilus HB27)

WVKILSVVGARPQFIKAAAVSRVLRASPGVREVLVHTGQHYDDNMSQVFF
EELEIPDPDYHLGIGGGTHGQNTGRMLEAIEGVLLKEKPDWVLVYGNTDS
TLAGALAAVKLHIPVAHVEAGLRSFNRRMPEEINRILTDHASDLLFAPTE
TAVQNLLREGIPENRIHLVGDVMYDAALHYGAKAERKSRILERLGLQAKG
YVLATIHRAENTDDQERLRVILEALAEVHQEVPVVFPVHPRTRKRAEAFG
LGSYLEKVVALEPVGYLDMVMLEKNARLIVTDSGGVQKEAYFYRVPCVTV
REETEWVELLKAEWNYLAAPQNAKDLALTILHRMRTKGVEIDLYGDGRAS
QKISDFLRKVG

3D structure

• PDB: 8sy9 Structural analysis of a bacterial UDP-sugar 2-epimerase reveals the active site architecture before and after catalysis.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.14: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MJL	B	R12 P13 Q14 I16 K17 G97 N98 T99 E120 E132 H208 R209 V265 Y267 M270 S284 G285 G286 E290 W307	R11 P12 Q13 I15 K16 G96 N97 T98 E119 E131 H207 R208 V264 Y266 M269 S283 G284 G285 E289 W306
BS02	UD1	B	P13 Q40 H41 Y42 D43 G67 T99 D100 E132 H208 R209 H240 P241 R242	P12 Q39 H40 Y41 D42 G66 T98 D99 E131 H207 R208 H239 P240 R241

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003824 catalytic activity
• GO:0008761 UDP-N-acetylglucosamine 2-epimerase activity
• GO:0016853 isomerase activity

External links

• PDB: RCSB:8sy9, PDBe:8sy9, PDBj:8sy9
• PDBsum: 8sy9
• PubMed: 37660908
• UniProt: Q72KY0