Structure of PDB 8sdb Chain B

Receptor sequence

>8sdbB (length=591) Species: 562 (Escherichia coli)

HLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHPMR
LRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETA
SLICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRE
LADQLVPYAKWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDD
FRYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTNLYEHSTLIYNYG
RREVSNFLVGNALYWIERFGIDALRVDAVASMIYRDYSNEFGGRENLEAI
EFLRNTNRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWM
HDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLPLSHDEVVHGKKSIL
DRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDASLDW
HLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPYGFEWLVVDDKER
SVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTDSMHY
HGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREA

3D structure

• PDB: 8sdb The Structure of Maltooctaose-Bound Escherichia coli Branching Enzyme Suggests a Mechanism for Donor Chain Specificity.
• Chain: B
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.18: 1,4-alpha-glucan branching enzyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	B	D496 V498	D360 V362
BS02	GLC	B	E635 T658 V660	E499 T522 V524
BS03	GLC	B	S583 L584	S447 L448
BS04	GLC	B	R254 R255 T257 S583	R137 R138 T140 S447
BS05	GLC	B	H256 T257 D258	H139 T140 D141
BS06	GLC	B	P469 W478	P333 W342

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003844 1,4-alpha-glucan branching enzyme activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0043169 cation binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005978 glycogen biosynthetic process

External links

• PDB: RCSB:8sdb, PDBe:8sdb, PDBj:8sdb
• PDBsum: 8sdb
• PubMed: 37298853
• UniProt: P07762|GLGB_ECOLI 1,4-alpha-glucan branching enzyme GlgB (Gene Name=glgB)