Structure of PDB 8rih Chain B

Receptor sequence

>8rihB (length=333) Species: 4932 (Saccharomyces cerevisiae)

SKIPIWLDCDPGHDDAIAILLGCFHPAFNLLGISTCFGNAPPENTDYNAR
SLLTAMGKAQAIPVYKGAQRPWKREPHYAPDIHGISGLDGTSLLPKPTFE
ARTDKTYIEAIEEAILANNGEISFVSTGALTTLATVFRCKPYLKKSVKYI
SIMGGGLHGLGNCNPNLSAEFNVWIDPDAANYIFRDPDVKDKCIVVPLNL
THKAIATYKVNEMIYNEKNNSKLRELFLELFQFFAHTYGFESGPPIHDPV
ASMPLLEFYGWDPSSAVGFRYKRMDISCIDDVFNENSGKIIIEKEYPNDS
DVGTIIGLDLNIQYFWDQIFEALNRADKMSTIG

3D structure

• PDB: 8rih Structure-Function Insights into the Dual Role in Nucleobase and Nicotinamide Metabolism and a Possible Use in Cancer Gene Therapy of the URH1p Riboside Hydrolase.
• Chain: B
• Resolution: 2.74 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	D13 D18 T130 G131 D255	D10 D15 T127 G128 D248

Gene Ontology

Molecular Function

• GO:0008477 purine nucleosidase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0045437 uridine nucleosidase activity
• GO:0050263 ribosylpyrimidine nucleosidase activity
• GO:0070635 nicotinamide riboside hydrolase activity
• GO:0070636 nicotinic acid riboside hydrolase activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006152 purine nucleoside catabolic process
• GO:0006216 cytidine catabolic process
• GO:0006218 uridine catabolic process
• GO:0008655 pyrimidine-containing compound salvage
• GO:0019358 nicotinate nucleotide salvage
• GO:0034356 NAD biosynthesis via nicotinamide riboside salvage pathway
• GO:0046135 pyrimidine nucleoside catabolic process

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8rih, PDBe:8rih, PDBj:8rih
• PDBsum: 8rih
• PubMed: 39000137
• UniProt: Q04179|URH1_YEAST Uridine nucleosidase (Gene Name=URH1)