Structure of PDB 8r3k Chain B

Receptor sequence

>8r3kB (length=740) Species: 1318616 (Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))

MDVNPTLLFLKVPVQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHKYS
EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP
GIFENSCLETMEIVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF
RSNGLTANESGRLIDFLKDVMDSMDKEEMEITTHFQRKRRVRDNMTKKMV
TQRTIGKKKQRLNKRSYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRG
FVYFVEALARSICEKLEQSGLPVGGNEKKAKLANVVRKMMTNSQDTELSF
TITGDNTKWNENQNPRMFLAMITYITRNQPEWFRNVLSIAPIMFSNKMAR
LGKGYMFESKSMKLRTQVPAEMLANIDLKYFNKSTREKIEKIRPLLIDGT
ASLSPGMMMGMFNMLSTVLGVSILNLGQKKYTKTTYWWDGLQSSDDFALI
VNAPNHEGIQAGVDRFYRTCKLVGINMSKKKSYINRTGTFEFTSFFYRYG
FVANFSMELPSFGVSGINESADMSVGVTVIKNNMINNDLGPATAQMALQL
FIKDYRYTYRCHRGDTQIQTRRAFELGKLWEQTRSKAGLLVSDGGPNLYN
IRNLHIPEVCLKWELMDEDYQGRLCNPMNPFVSHKEISMEYDAVATTHSW
IPKRNRSILNTSQRGILEDEQMYQKCCNLFEKFFPSSSYRRPVGISSMVE
AMVSRARIDARIDFESGRIKKEEFAEIMKICSTIEELRRQ

3D structure

• PDB: 8r3k The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome.
• Chain: B
• Resolution: 3.43 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	B	H32 T34 Y38 G125 R126 Q127 N136 Q186 K198 R203 M227 K229 I241 A242 T243 R249 R260 P272 G274 M356 R365 G410 N413 M414 V525 T528 N532 S633 I674 L675 N676 R680 L683 E686 Q687 Q690	H32 T34 Y38 G125 R126 Q127 N136 Q186 K198 R203 M227 K229 I241 A242 T243 R249 R260 P272 G274 M356 R365 G410 N413 M414 V525 T528 N532 S633 I658 L659 N660 R664 L667 E670 Q671 Q674
BS02	rna	B	Y24 Q124 R239 N310 M409 G410 D445 D446 M507 P510 R706	Y24 Q124 R239 N310 M409 G410 D445 D446 M507 P510 R690
BS03	POP	B	R239 N306 K308 W309 N310 K481	R239 N306 K308 W309 N310 K481
BS04	MG	B	D305 N306 D445	D305 N306 D445
BS05	MG	B	D305 D445 D446	D305 D445 D446

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0005737 cytoplasm
• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:8r3k, PDBe:8r3k, PDBj:8r3k
• PDBsum: 8r3k
• PubMed: 38316757
• UniProt: M9TLW3