Structure of PDB 8qmf Chain B

Receptor sequence

>8qmfB (length=674) Species: 196600 (Vibrio vulnificus YJ016)

MDRKHLANAIRALSMDGVQKANSGHPGAPMGMADIAEVLWRGHLNHNPSN
PEWADRDRFVLSNGHGSMLIYSLLHLSGYELSIDDLKNFRQLHSKTPGHP
EYGYAPGIETTTGPLGQGITNAVGMAMAEKALAAQFNKEGHDIVDHFTYV
FMGDGCLMEGISHEACSLAGTLGLGKLIAFWDDNGISIDGHVEGWFSDDT
PKRFEAYGWHVIPAVDGHNAEAINAAIEAAKADPRPTLICTKTIIGFGSP
NKSGSHDCHGAPLGAEEIAATRKELGWEHGPFEIPQEVYAEWSAKEAGAA
KEAAWNEKFAAYEAAYPELAAEFKRRVNGELPAQWEEKANQIIADLQANP
ANIASRKASQNALEAFGKMLPEFMGGSADLAPSNLTMWSGSKSLEASDFS
GNYIHYGVREFGMTAIMNGIALHGGFVPYGATFLMFMEYARNAMRMAALM
KVQNIQVYTHDSIGLGEDGPTHQPVEQIASLRLTPNMSTWRPCDQVESAV
AWKLAIERKDGPSALIFSRQNLAQQERTAEQVADIAKGGYILKDSDGKPE
LILIATGSEVELAVKAAEQLTAEGKKVRVVSMPATDAFDKQDAAYRESVL
PSDVTARIAIEAGITDFWYKYVGFDGRIIGMTTFGESAPADQLFEMFGFT
VENVVNTAKELLAENLYFQGLEHH

3D structure

• PDB: 8qmf Structural determination and kinetic analysis of the Transketolase from Vibrio vulnificus reveal unexpected cooperative behavior.
• Chain: B
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 2.2.1.1: transketolase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	TPP	B	D379 E410 F436 Y439	D379 E410 F436 Y439
BS02	TPP	B	H65 G113 L115 D154 G155 N184 I186 I188 I245 H259	H65 G113 L115 D154 G155 N184 I186 I188 I245 H259
BS03	MG	B	D154 N184 I186	D154 N184 I186

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004802 transketolase activity
• GO:0016740 transferase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006098 pentose-phosphate shunt

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:8qmf, PDBe:8qmf, PDBj:8qmf
• PDBsum: 8qmf
• PubMed: 38145310
• UniProt: Q7MDD4|TKT2_VIBVY Transketolase 2 (Gene Name=tkt2)