Structure of PDB 8qa6 Chain B

Receptor sequence

>8qa6B (length=553) Species: 9606 (Homo sapiens) [Search protein sequence]

RLREKMRRRLESGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDV
TWHPAGDPGSDKETSSMMIASTAVNYCGLETILHMTCCRQRLEEITGHLH
KAKQLGLKNIMALRGDPGFNYAVDLVKHIRSEFGDYFDICVAGYPKGHPE
AGSFEADLKHLKEKVSAGADFIITQLFFEADTFFRFVKACTDMGITCPIV
PGIFPIQGYHSLRQLVKLSKLEVPQEIKDVIEPIKDNDAAIRNYGIELAV
SLCQELLASGLVPGLHFYTLNREMATTEVLKRLGMWTEEWDEFPNGRWGN
SSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEASVFEVFVLYLSG
EPNRNGHKVTCLPWNDEPLAAETSLLKEELLRVNRQGILTINSQPNINGK
PSSDPIVGWGPSGGYVFQKAYLEFFTSRETAEALLQVLKKYELRVNYHLV
NVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALW
IEQWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVEDTLE
LLN

3D structure

PDB

8qa6 Dynamic inter-domain transformations mediate the allosteric regulation of human 5, 10-methylenetetrahydrofolate reductase

Chain

Resolution

2.91 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

1.5.1.53: methylenetetrahydrofolate reductase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	SAM	B	N290 D291 F384 N386 I570 P572	N237 D238 F293 N295 I479 P481
BS02	FAD	B	W95 H127 L156 R157 G158 D159 Y174 A175 A195 Y197 H201 A204 D210 H213 K217 Y404	W52 H84 L113 R114 G115 D116 Y121 A122 A142 Y144 H148 A151 D157 H160 K164 Y313
BS03	SAM	B	W389 Y438 L439 N456 A461 E463 T464 L471 T481 I482 N483 S484 Q485 Q509 T573	W298 Y347 L348 N365 A370 E372 T373 L380 T390 I391 N392 S393 Q394 Q418 T482

Gene Ontology

	Molecular Function
GO:0004489	methylenetetrahydrofolate reductase (NAD(P)H) activity
GO:0005515	protein binding
GO:0016491	oxidoreductase activity
GO:0044877	protein-containing complex binding
GO:0050660	flavin adenine dinucleotide binding
GO:0050661	NADP binding
GO:0071949	FAD binding
GO:0072341	modified amino acid binding
GO:0106313	methylenetetrahydrofolate reductase (NADPH) activity

	Biological Process
GO:0001666	response to hypoxia
GO:0001843	neural tube closure
GO:0006555	methionine metabolic process
GO:0009086	methionine biosynthetic process
GO:0009410	response to xenobiotic stimulus
GO:0033274	response to vitamin B2
GO:0035999	tetrahydrofolate interconversion
GO:0043200	response to amino acid
GO:0046500	S-adenosylmethionine metabolic process
GO:0046653	tetrahydrofolate metabolic process
GO:0050667	homocysteine metabolic process
GO:0051593	response to folic acid
GO:0070555	response to interleukin-1
GO:0070828	heterochromatin organization

	Cellular Component
GO:0005829	cytosol

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8qa6, PDBe:8qa6, PDBj:8qa6
PDBsum	8qa6
PubMed	38622112
UniProt	P42898\|MTHR_HUMAN Methylenetetrahydrofolate reductase (NADPH) (Gene Name=MTHFR)

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