Structure of PDB 8q4h Chain B

Receptor sequence

>8q4hB (length=503) Species: 884048 (Desulfitobacterium hafniense TCE1)

TSEFPYKVDAKYQRYNSLKNFFEKTFDPEANKTPIKFHYDDVSKITGKKD
TGKDLPTLNAERLGIKGRPATHTETSILFHTQHLGAMLTQRHNETGWTGL
DEALNAGAWAVEFDYSGFNATGGGPGSVIPLYPINPMTNEIANEPVMVPG
LYNWDNIDVESVRQQGQQWKFESKEEASKIVKKATRLLGADLVGIAPYDE
RWTYSTWGRKIYKPCKMPNGRTKYLPWDLPKMLSGGGVEVFGHAKFEPDW
EKYAGFKPKSVIVFVLEEDYEAIRTSPSVISSATVGKSYSNMAEVAYKIA
VFLRKLGYYAAPCGNDTGISVPMAVQAGLGEAGRNGLLITQKFGPRHRIA
KVYTDLELAPDKPRKFGVREFCRLCKKCADACPAQAISHEKDPKVLQPED
CEVAENPYTEKWHLDSNRCGSFWAYNGSPCSNCVAVCSWNKVETWNHDVA
RIATQIPLLQDAARKFDEWFGYNGPVNPDERLESGYVQNMVKDFWNNPES
IKQ

3D structure

• PDB: 8q4h Structure of a membrane-bound menaquinol:organohalide oxidoreductase
• Chain: B
• Resolution: 2.83 Å

Enzymatic activity

• Enzyme Commision number: 1.21.99.5: tetrachloroethene reductive dehalogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	JYF	B	W157 Y337 W471	W109 Y289 W423
BS02	COB	B	F52 Y54 Y63 Y252 N363 P370 G384 L385 H395 R396 I397 A452 E453 T457 K459 L462 W471	F4 Y6 Y15 Y204 N315 P322 G336 L337 H347 R348 I349 A404 E405 T409 K411 L414 W423
BS03	SF4	B	R382 N383 C420 C423 K425 C426 C485 W487	R334 N335 C372 C375 K377 C378 C437 W439
BS04	SF4	B	C430 C467 C478 C481	C382 C419 C430 C433
BS05	MQ7	B	S486 W487 K489 H495 A498 T502 A511 F514 D515 F518	S438 W439 K441 H447 A450 T454 A463 F466 D467 F470

Gene Ontology

Molecular Function

• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

• GO:0005886 plasma membrane

External links

• PDB: RCSB:8q4h, PDBe:8q4h, PDBj:8q4h
• PDBsum: 8q4h
• PubMed: 37923808
• UniProt: Q8GJ31|PCEA2_DESHA Tetrachloroethene reductive dehalogenase (Gene Name=pceA)