Structure of PDB 8oto Chain B

Receptor sequence

>8otoB (length=116) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

AFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQES
FGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVC
TVCGMWKGYGCSCDQL

3D structure

• PDB: 8oto Crystal structures of Tubercidin and Adenosine bound to the active site of the SARS-CoV-2 methyltransferase nsp10-16
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C4327 C4330 H4336 C4343	C57 C60 H66 C73
BS02	ZN	B	C4370 C4373 C4381 C4383	C100 C103 C111 C113

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0008270 zinc ion binding

Biological Process

• GO:0019079 viral genome replication

External links

• PDB: RCSB:8oto, PDBe:8oto, PDBj:8oto
• PDBsum: 8oto
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)