Structure of PDB 8k9e Chain B

Receptor sequence

>8k9eB (length=951) Species: 324602 (Chloroflexus aurantiacus J-10-fl)

CTYQPRQYIAPFDRQPEGRVPGIPQYFASTLTLGGYGTGVLVRSNEGRPT
KVEGNPRHPASLGGTDLFAQAEILTMYDPDRSTTVLRQGVPSTWAEFTTT
LGNALTAARATQGAGVRLLTTTITSPSLAAQIEQFLQAYPQARWYQYEPI
NRDNVVAGARLAFGRDVTTRYDLSAAQVVVSLDADFLAPGPGFVAYARAF
AERRKVRKDSTTMNRLYVVEASPSTTGTAADHRLPLRADAIAAFTGALAN
ELGVGGAPATLSPKAEEFLRAIARDLEEHRGQSVVIAGDQQPPIVHALAH
LINAELGNVGQTVFYHEPVEARPTNQTEELVALVSEMAAGRVETLIMIGG
NPVYNAPGDLRFADRMASVPLTIHLSQFVDETSARATWHIPQAHPLESWG
DARAFDGTASIVQPLIEPLYGGKTANELLAAMLGQPEAESYDLVRSFWLE
QIGETGWQVALANGVIAETVAPVIEPTLNEGAIRATPIPQPGDGVEIVFR
PDPSLFDGFYANNGWLQELPRPLTKLVWDNAALMSPRTAIKLLGLPFNAD
RLIGTEADDRERQQYLEQLSKVNGTIARIEYRGGIIEIPIWLLPGHAEDS
ITLNLGYGRTHAGRVGNNVGIDVYPIRTSDSPWFGAGARVTNTGRTYLLV
STQDHWTLEGRDIYRVGEFKKFKEDPKYIAKEVYQEEYGRETPNYQSLQP
GDDYTGRNAWGMTINLNACIGCNACVVACQAENNIAVVGKDQVSRGREMH
WIRIDRYFAGEDLDNPSIYMMPVNCMQCEKAPCEVVCPVAATVHDYEGLN
NMVYNRCVGTKYCSNNCPYKVRRFNFLQYSDTTTETFKLAFNPDVTVRIR
GVMEKCTYCVQRISGARIAAKRAAVQAGQSSYVISDGAIQTACEQACPTG
AIVFGDINDSNSRVAKWKAEGHNYGLLGFLNTVPRTTYLARVRNPSEELE
K

3D structure

• PDB: 8k9e Cryo-EM structure of HQNO-bound Alternative Complex III from the anoxygenic phototrophic bacterium Chloroflexus aurantiacus.
• Chain: B
• Resolution: 3.33 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	B	Y78 N880	Y3 N805
BS02	HEC	B	R942 K946	R867 K871
BS03	SF4	B	C794 I795 G796 C797 N798 C800 I829 C972 T974	C719 I720 G721 C722 N723 C725 I754 C897 T899
BS04	SF4	B	C804 N808 C931 Y933 C934 T966 C968	C729 N733 C856 Y858 C859 T891 C893
BS05	SF4	B	C850 M851 C853 P857 C858 N875 C892 Y894 V896 R897	C775 M776 C778 P782 C783 N800 C817 Y819 V821 R822
BS06	F3S	B	C862 M877 C882 V883 G884 T885 K886 Y887 C888 M928	C787 M802 C807 V808 G809 T810 K811 Y812 C813 M853

External links

• PDB: RCSB:8k9e, PDBe:8k9e, PDBj:8k9e
• PDBsum: 8k9e
• PubMed: 38299372
• UniProt: A9WEV3