Structure of PDB 8jt1 Chain B

Receptor sequence

>8jt1B (length=532) Species: 673 (Grimontia hollisae)

QCDLSQFQTTSSNQLMAAIRQQGASCVNALFSADTGVQEAAFSSNHMYNV
AQYTRTLAQQYAGGGSDELEALYLYLRAGYYAEFYNSNITFLSWVTPAVK
GAVDAFVQNAHFYDNGDAHGKVLNEVIITMDSAGLQHAYLDVVTQWLTRW
NAQYAEHWYMRNAVNGVFTLLFGGQWNNQYTSLIGEQTALVTALQAFALD
RTKVNSPTEFMAANAARELGRLARYTDATIAPKVTEGLTAIFGQYPSYGD
GDAIWLGAADTASYYADCSQFNICGFEDALRDAALNQTFICSDTIKIRSQ
DMSQAQHLAACDKMAYEESFFHTTLETGNQPVADDHNTQLQVNIFNSDTD
YGKYAGPIFGIDTNNGGMYLEGNPANVGNIPNFIAYEASYANPDHFVWNL
EHEYVHYLDGRFNMYGDFGTPTELVVWWSEGVAEYVSRVNDNPQAIATIQ
DGSTYTLAQVFDTTYDGFDVDRIYRWGYLAVRFMFERHPDEVQRMLSATR
QGRWAEYKAIISGWANQYQSEFAQWTEALAKG

3D structure

• PDB: 8jt1 Insights into the catalytic mechanism of Grimontia hollisae collagenase through structural and mutational analyses.
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.3: microbial collagenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	B	N455 G456 G457 M458 Y459 W488 N489 E493 H496 F508 E520 Y555 Y564	N365 G366 G367 M368 Y369 W398 N399 E403 H406 F418 E430 Y465 Y474
BS02	peptide	B	N118 F121 R167 Y171 F174 E215 I218 D221 S222	N28 F31 R77 Y81 F84 E125 I128 D131 S132
BS03	peptide	B	F258 F262 N304 R307 E308 R311	F168 F172 N214 R217 E218 R221
BS04	ZN	B	H492 H496 E520	H402 H406 E430
BS05	CA	B	E461 G500 M504 G506	E371 G410 M414 G416
BS06	CA	B	D391 N436 E477	D301 N346 E387
BS07	CA	B	T538 D541 S543	T448 D451 S453
BS08	CA	B	E173 N176 S177 I179	E83 N86 S87 I89

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity
• GO:0008270 zinc ion binding

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:8jt1, PDBe:8jt1, PDBj:8jt1
• PDBsum: 8jt1
• PubMed: 37698340
• UniProt: F7IZI6