Structure of PDB 8jsi Chain B

Receptor sequence

>8jsiB (length=733) Species: 277988 (Thermococcus thioreducens)

MLMKVLTNMVKLNQDIIPNEIYLYKIFNKPEDGMNIYKIAYRNHGIVIDP
QNRIIATPSELEYSGKFAIEDEISFNELPENYQNRLVLRILRDNGISDHA
LSRTLQKYRKPKPFGDFEVIPEIRSSVIKHGGDFYLVLHLSHQIRSKKTL
WELVGRNKDALRDFLKEHRGTILLRDIASEHKVVYKPIFKRYNGDPDLIE
DNSNDVEHWYDYHLERYWNTPELKKEFYKKFGPVDLNQPIILAKPLRQHN
RGDLVHLLPQFVVPVYNAEQLNDILASEILEYLKLTSNQRISLLSRLIND
IKTNTNIIVSSLTELEANTFDVDLNDMLQVRNADNVKVTLSELEISKTRL
FTWMKSRKYPVILPYDIPQKLKKIEKIPVFIIVDSALSRDIQTFAKDEFR
YLISSLQKSLSNWVDFPILDIRDKYIFTIDLTSDKDIVNLSIKLVNLMKN
AELGLALIATRTKLPNETFDEVKKRLFSVNIISQVVNEATLYKRDKYNES
RLNLYVQHNLLFQILSKLGIKYYVLRHKFSYDYIVGIDVTPMKLSHGYIG
GSAVMFDSIRKIIPVEIGEQMGESIDMKEFFKDMVVQFGKFGIDLEGKSI
LILRDGKITKDEEEGLAYISKVFGIKITTFNIVKRHLLRIFANRKLYLRL
ANSVYLLPHRIKQSVGTPVPLKLSEKRLILDGTITSQEITYNDIFEILLL
SELNYGSISADMKLPAPVHYAHKFVRALRKGWR

3D structure

• PDB: 8jsi Cryo-EM structure of a DNA-protein complex
• Chain: B
• Resolution: 2.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	Y37 Y41 R85 R92 D98 A268 L280 T348 R349 K463 Y505 D538 T540 V636 R638 Q666 K675 R729 R732	Y37 Y41 R85 R92 D98 A268 L280 T348 R349 K463 Y505 D538 T540 V633 R635 Q663 K672 R726 R729
BS02	dna	B	Y37 D49 P50 Q51 Y82 S141 H142 Q143 R145 R175 V184 L246 R247 Q248 K284 R290 T462 K463 L464 V485 V486 N487 T490 V506 N509 Q513 Y548 T612 K613 H662 I664 Q666 T670 M715 K726	Y37 D49 P50 Q51 Y82 S141 H142 Q143 R145 R175 V184 L246 R247 Q248 K284 R290 T462 K463 L464 V485 V486 N487 T490 V506 N509 Q513 Y548 T609 K610 H659 I661 Q663 T667 M712 K723
BS03	ZN	B	H249 H546	H249 H546
BS04	MG	B	D538 H725	D538 H722

External links

• PDB: RCSB:8jsi, PDBe:8jsi, PDBj:8jsi
• PDBsum: 8jsi
• UniProt: A0A0Q2M2Z1