Structure of PDB 8jaz Chain B

Receptor sequence

>8jazB (length=479) Species: 1328314 (Azotobacter chroococcum NCIMB 8003)

MDFDVKDFGAKGDGKSDDTEAIQAAIDAAYEAGGGTVRLSAGEYRVSGGD
EASDGALMIKSNVYMDGAGMGETVIKLVDGWDQKLTGIIRSKNGEKTHDY
GIRDLTLDGNQDNTEGEVDGFYTGYIPREDGADYNVTAERVEIREVSRYG
FDPHEQTINLTIRDSVAHNNGKDGFVGDFQIDSTFENNVSHDNGRHGFNI
VTSSHDILLRDNVAYGNGANGLVVQRGSEDIAHPYNIQIEGGAYHDNGAE
GVLIKMTSNASLQGAEIYGNDAAGVRVRGVDGMQLLDNDIHDNAQGGGKA
EIVLEDYDDRDGVSGNYYETLNATVQGNRVAGAAQLLSSEGRDLLDGAAG
NDLLDGGAGRDTLSGGGGADTFRFADRQDSFRNYEGDTSRVDDIVDFTPG
ADLIDLSGLGYSGLGDGYNGTLALLLNEDGTKTYLKDRQADAQGNHFEIA
LDGNLVDSLSATDIAFDATQLELLGTTDL

3D structure

• PDB: 8jaz Structural basis for the minimal bifunctional alginate epimerase AlgE3 from Azotobacter chroococcum.
• Chain: B
• Resolution: 1.82 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.37: mannuronan 5-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BEM	B	R195 N199 S228	R195 N199 S228
BS02	BEM	B	R195 H196 Q225 G227 X255	R195 H196 Q225 G227 X255
BS03	CA	B	S91 X92 E95 T97 G124 D133	S91 X92 E95 T97 G124 D133
BS04	CA	B	S339 G341 D343 G356 A358 D361	S339 G341 D343 G356 A358 D361
BS05	CA	B	G357 G359 D361 D379 D392	G357 G359 D361 D379 D392
BS06	CA	B	A348 G350 D352 G365 G367 D370	A348 G350 D352 G365 G367 D370
BS07	CA	B	G366 G367 G368 D370 D402	G366 G367 G368 D370 D402
BS08	CA	B	D66 G67	D66 G67

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding
• GO:0016829 lyase activity
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0042121 alginic acid biosynthetic process

External links

• PDB: RCSB:8jaz, PDBe:8jaz, PDBj:8jaz
• PDBsum: 8jaz
• PubMed: 38649293
• UniProt: A0A0C4WKK2