Structure of PDB 8j9w Chain B

Receptor sequence

>8j9wB (length=775) Species: 10497 (African swine fever virus) [Search protein sequence]

YTRARNAGGKRAQDCMLLAAEGDSALSLLRTGLTLGKSNPSGPSFDFCGM
ISLGGVIMNACKKVTNITTDSGETIMVRNEQLTNNKVLQGIVQVLGLDFN
CHYKTQEERAKLRYGCIVACVDQDLDGCGKILGLLLAYFHLFWPQLIIHG
FVKRLLTPLIRVYEKGKTMPVEFYYEQEFDAWAKKQTSLVNHTVKYYKGL
AAHDTHEVKSMFKHFDNMVYTFTLDDSAKELFHIYFGGESELRKRELCTG
VVPLTETQTQSIHSVRRIPCSLHLQVDTKAYKLDAIERQIPNFLDGMTRA
RRKILAGGVKCFASNNRERKVFQFGGYVADHMFYHHGDMSLNTSIIKAAQ
YYPGSSHLYPVFIGIGSFGSRHLGGKDAGSPRYISVQLASEFIKTMFPAE
DSWLLPYVFEDGQRAEPEYYVPVLPLAIMEYGANPSEGWKYTTWARQLED
ILALVRAYVDKDNPKHELLHYAIKHKITILPLRPSNYNFKGHLKRFGQYY
YSYGTYDISEQRNIITITELPLRVPTVAYIESIKKSSNRMTFIEEIIDYS
SSETIEILVKLKPNSLNRIVEEFKETEEQDSIENFLRLRNCLHSHLNFVK
PKGGIIEFNSYYEILYAWLPYRRELYQKRLMREHAVLKLRIIMETAIVRY
INESAELNLSHYEDEKEASRILSEHGFPPLNHTLIISPEFASIEELNQKA
LQGCYTYILSLQARELLIAAKTRRVEKIKKMQARLDKVEQLLQESPFPGA
SVWLEEIDAVEKAIIKGRNTQWKFH

3D structure

PDB

8j9w Cryo-EM structure of the African swine fever virus topoisomerase 2 complexed with Cut02bDNA and etoposide (EDI-2)

Chain

Resolution

2.76 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	B	V473 T482 E497 D543 R705 Q706 T715 A717 Y751 H753 S761 K793 N851	V56 T65 E80 D126 R288 Q289 T298 A300 Y334 H336 S344 K376 N434
BS02	dna	B	D440 M756	D23 M339
BS03	dna	B	M475 N476 K479 K480 Q498 S657 P852 S853 E854 G855 W856 K857 H1010	M58 N59 K62 K63 Q81 S240 P435 S436 E437 G438 W439 K440 H593
BS04	EVP	B	E438 G439 D440 S757	E21 G22 D23 S340

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003916	DNA topoisomerase activity
GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524	ATP binding
GO:0046872	metal ion binding

	Biological Process
GO:0000819	sister chromatid segregation
GO:0006259	DNA metabolic process
GO:0006265	DNA topological change

	Cellular Component
GO:0030430	host cell cytoplasm

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8j9w, PDBe:8j9w, PDBj:8j9w
PDBsum	8j9w
PubMed	38418525
UniProt	Q00942\|TOP2_ASFB7 DNA topoisomerase 2 (Gene Name=TOP)

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